Jan 16, 2016

When Wavelengths Collide: Bias in Cell Abundance Measurements due to Expressed Fluorescent Proteins

BioRxiv : the Preprint Server for Biology
Ariel HechtMatthew S. Munson

Abstract

The abundance of bacteria in liquid culture is commonly inferred by measuring optical density at 600 nm. Red fluorescent proteins (RFP) can strongly absorb light at 600 nm. Increasing RFP expression can falsely inflate apparent cell density and lead to underestimations of mean per-cell fluorescence by up to 10%. Measuring optical density at 700 nm would allow estimation of cell abundance unaffected by the presence of nearly all fluorescent proteins.

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Mentioned in this Paper

Red fluorescent protein
Fluorescent stain
Microscopy
Laboratory Culture
Protein Expression

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