White adipose tissue urea cycle activity is not affected by one-month treatment with a hyperlipidic diet in female rats

Food & Function
Sofía ArriaránJosé Antonio Fernández-López

Abstract

Under high-energy diets, amino acid N is difficult to dispose of, as a consequence of the availability of alternative substrates. We found, recently, that WAT contains a complete functional urea cycle, we analyzed the possible overall changes in the WAT urea cycle (and other-related amino acid metabolism gene expressions) in rats subjected to a cafeteria diet. Adult female Wistar rats were fed control or simplified cafeteria diets. Samples of WAT sites: mesenteric, periovaric, retroperitoneal and subcutaneous, were used for the estimation of all urea cycle enzyme activities and gene expressions. Other key amino acid metabolism gene expressions, and lactate dehydrogenase were also measured. Subcutaneous WAT showed a differentiated amino acid metabolism profile, since its cumulative (whole site) activity for most enzymes was higher than the activities of the other sites studied. After one month of eating an energy-rich cafeteria diet, and in spite of doubling the size of WAT, the transforming capacity of most amino acid metabolism enzymes remained practically unchanged in the tissue. This was not only due to limited changes in the overall enzyme activity, but also a consequence of a relative decrease in the expression of the corr...Continue Reading

References

Dec 1, 1979·Metabolism: Clinical and Experimental·J OstmanL Kager
Aug 8, 1977·Biochemical and Biophysical Research Communications·K Snell, D A Duff
Sep 1, 1976·Physiology & Behavior·A Sclafani, D Springer
Feb 1, 1989·Physiology & Behavior·E PratsM Alemany
Sep 15, 1985·The Biochemical Journal·T BarberJ Cabo
Mar 1, 1969·The Journal of Clinical Investigation·P FeligG F Cahill
Nov 1, 1982·Archives of Biochemistry and Biophysics·J G Henslee, M E Jones
Jul 1, 1994·Physiology & Behavior·M EsteveM Alemany
Jan 1, 1996·The American Journal of Physiology·M M Briggs, F Schachat
Oct 1, 1996·Baillière's Clinical Endocrinology and Metabolism·P TessariA Tiengo
Oct 30, 2001·The Proceedings of the Nutrition Society·K N Frayn
Aug 2, 2003·Biochemical and Biophysical Research Communications·Fabrice BertileThierry Raclot
Jun 7, 2005·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S Cinti
Jul 20, 2006·Annual Review of Nutrition·George F Cahill
Mar 24, 2007·Metabolism: Clinical and Experimental·Ariane BoivinAndré Tchernof
Apr 10, 2008·The British Journal of Nutrition·Paul TrayhurnI Stuart Wood
Dec 23, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·M B Zimmermann, I Aeberli
Jan 23, 2010·The Journal of Biological Chemistry·Mark A HermanBarbara B Kahn
Aug 14, 2010·Mediators of Inflammation·Rosário Monteiro, Isabel Azevedo
May 7, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Chiadi E NdumeleRaul D Santos
Feb 9, 2012·Nutrition Research Reviews·Marià Alemany
Oct 6, 2012·Current Opinion in Clinical Nutrition and Metabolic Care·Gijs H Goossens, Ellen E Blaak
Jan 1, 2013·Diabetes·Leanne HodsonKeith N Frayn
Oct 30, 2013·International Journal of Obesity : Journal of the International Association for the Study of Obesity·K N Frayn, F Karpe
Jan 10, 2014·The Journal of Endocrinology·Haiming Cao
Jan 29, 2014·European Journal of Endocrinology·Antonio GiordanoSaverio Cinti
Jun 20, 2014·Experimental and Therapeutic Medicine·Marjan MotieGregg C Fonarow
Jul 22, 2014·International Journal of Obesity : Journal of the International Association for the Study of Obesity·H CastroA Palou

❮ Previous
Next ❯

Citations

Oct 23, 2020·International Journal of Molecular Sciences·Xavier Remesar, Marià Alemany

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.