Whole animal in vivo imaging after transient, nonviral gene delivery to the rat central nervous system.

Molecular Therapy : the Journal of the American Society of Gene Therapy
Ellen S HauckJames G Hecker

Abstract

We previously showed that a vector:lipid delivery system, comprised of a plasmid DNA vector and cationic lipid (lipoplex), when injected into the cerebrospinal fluid (CSF) of rats can deliver reporter genes in vivo efficiently and with widespread expression to the Central Nervous System (CNS). To further characterize this delivery system, we now present experiments that demonstrate the in vivo time-to-peak expression of the reporter gene, firefly luciferase. We infused a formulated lipoplex containing the lipid MLRI [dissymmetric myristoyl (14:0) and lauroyl (12:1) rosenthal inhibitor-substituted compound formed from the tetraalkylammonium glycerol-based DORI] and pNDluc, a luciferase vector, into CSF in the cisterna magna (CM) of the rat. Luciferase activity was followed over time by bioluminescence imaging after injection of luciferin. Our results show that luciferase activity in the CNS of rats is widespread, peaks 72 hours after injection into CM and can be detected in vivo for at least 7-10 days after peak expression. We further show that in contrast to injection into CSF, enzyme activity is not widely distributed after injection of the vector into brain parenchyma, emphasizing the importance of CSF delivery to achieve wid...Continue Reading

References

Mar 29, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·J G HeckerV R Irion
Dec 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·S Bhaumik, S S Gambhir
Sep 28, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Edmund J NiedzinskiMichael H Nantz
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bakhos A TannousXandra O Breakefield
Feb 7, 2006·Current Gene Therapy·John R OhlfestDavid A Largaespada
Apr 21, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Arthur W NienhuisBrian P Sorrentino
Apr 28, 2006·Nature·Niels-Bjarne WoodsInder M Verma
May 3, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Piotr HadaczekKrystof Bankiewicz
Jun 2, 2006·Analytical Biochemistry·Garrett R RettigKevin G Rice
Apr 6, 2007·The AAPS Journal·Xiang GaoDexi Liu
Jun 20, 2007·Journal of Cellular Physiology·Terence R Flotte
Jul 21, 2007·Journal of Neurosurgery·Seunguk OhJohn R Ohlfest
Nov 13, 2007·Journal of Biomedical Optics·Tewfik MiloudGünter J Hämmerling

❮ Previous
Next ❯

Citations

Sep 26, 2008·International Archives of Medicine·Ti-Fei Yuan, Oscar Arias-Carrión
Feb 24, 2009·Advanced Drug Delivery Reviews·Jian WuNipavan Chiamvimonvat
Nov 17, 2016·Frontiers in Molecular Neuroscience·James-Kevin Y TanPhilip J Horner
Mar 7, 2020·Advanced Drug Delivery Reviews·M J FowlerR W Sirianni
Jan 19, 2010·International Journal of Pharmaceutics·S ZouJ G Hecker
Mar 6, 2010·Tetrahedron Letters·Souvik BiswasMichael H Nantz

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.

© 2022 Meta ULC. All rights reserved