Whole blood assays to identify Th1 cell antigens and peptides encoded by Mycobacterium tuberculosis-specific RD1 genes

Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre
Shumaila N M HanifAbu Salim Mustafa

Abstract

To identify Th1 cell-stimulating antigens/peptides encoded by the genes predicted in the Mycobacterium tuberculosis-specific genomic region of difference (RD)1, deleted in Mycobacterium bovis Bacille Calmette-Guérin(BCG), by using synthetic peptides and whole blood from tuberculosis (TB) patients. Heparinized peripheral blood was obtained from culture-proven pulmonary TB patients (n = 16) attending the Chest Disease Hospital, Kuwait. Whole blood was diluted with tissue culture medium RPMI-1640 and tested for Th1 cell stimulation using antigen-induced proliferation and interferon-gamma (IFN-gamma) secretion assays. The antigens included a peptide pool of 220 peptides covering the sequence of 12 open reading frames (ORFs) of RD1 (RD1(mix)), peptide pools of RD1 ORF5 (ORF5(mix)), ORF6 (ORF6(mix)) and ORF7 (ORF7(mix)), and individual peptides of ORF6 (P6.1-P6.6) and ORF7 (P7.1-P7.6). M. tuberculosis culture filtrate, cell walls and whole-cell M. bovis BCG were used as complex mycobacterial antigens. The results obtained with different antigens and peptides were statistically analyzed for significant differences using Z test. The complex mycobacterial antigens (culture filtrate, cell walls and M.bovis BCG) and RD1(mix) induced compa...Continue Reading

References

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May 3, 2005·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Abu S MustafaHanady A Amoudy
Aug 17, 2005·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·A S Mustafa
Feb 18, 2006·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Hanady A AmoudyAbu S Mustafa
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Citations

Apr 11, 2001·Journal of Inorganic Biochemistry·I EndoM Odaka
Aug 5, 2017·International Journal of Mycobacteriology·Shumaila N M Hanif, A S Mustafa
Nov 3, 2010·Scandinavian Journal of Immunology·S N M HanifA S Mustafa
Aug 5, 2011·Scandinavian Journal of Immunology·S N M HanifA S Mustafa
Jan 20, 2011·Microbiology and Immunology·Abu Salim MustafaRajaa Al-Attiyah
May 19, 2020·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·Abu Salim Mustafa

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