Whole blood gene expression profiles in insulin resistant Latinos with the metabolic syndrome

PloS One
Samantha E TangenDawn K Coletta

Abstract

Although insulin resistance in skeletal muscle is well-characterized, the role of circulating whole blood in the metabolic syndrome phenotype is not well understood. We set out to test the hypothesis that genes involved in inflammation, insulin signaling and mitochondrial function would be altered in expression in the whole blood of individuals with metabolic syndrome. We further wanted to examine whether similar relationships that we have found previously in skeletal muscle exist in peripheral whole blood cells. All subjects (n=184) were Latino descent from the Arizona Insulin Resistance registry. Subjects were classified based on the metabolic syndrome phenotype according to the National Cholesterol Education Program's Adult Treatment Panel III. Of the 184 Latino subjects in the study, 74 were classified with the metabolic syndrome and 110 were without. Whole blood gene expression profiling was performed using the Agilent 4x44K Whole Human Genome Microarray. Whole blood microarray analysis identified 1,432 probes that were altered in expression ≥ 1.2 fold and P<0.05 after Benjamini-Hochberg in the metabolic syndrome subjects. KEGG pathway analysis revealed significant enrichment for pathways including ribosome, oxidative phos...Continue Reading

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Citations

Jan 21, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Samantha E DayDawn K Coletta
Aug 22, 2018·Neural Regeneration Research·Paige E Herman, Ona Bloom

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Datasets Mentioned

BETA
E-MEXP-12345

Methods Mentioned

BETA
electrophoresis
Feature Extraction
PCR
enzyme-linked immunosorbent assay

Software Mentioned

TRANSFAC
GeneSpring
SPSS
PScan
Feature Extraction
DAVID
JASPAR

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