Whole-body kinetic image of a redox probe in mice using Overhauser-enhanced MRI

Free Radical Biology & Medicine
Nuttavut KosemHideo Utsumi

Abstract

Overhauser-enhanced MRI (OMRI) enables visualization of free radicals in animals based on dynamic nuclear polarization. Real-time data of tissue redox status gathered from kinetic images of redox-sensitive nitroxyl radical probes using OMRI provided both anatomic and physiological information. Phantom experiments demonstrated the linear correlation between the enhancement factor and the concentration of a membrane-impermeable probe, carboxy-PROXYL (3-carboxy-2,2,5,5-tetramethyl- pyrrolidine-1-oxyl). Whole-body OMRI images illustrated the in vivo kinetics of carboxy-PROXYL for 25 min. Initial distribution was observed in lung, heart, liver, and kidney, but not brain, corresponding to its minimal lipophilicity. Based on these images (pixel size, 1.33 × 1.33 mm; slice thickness, 50mm), a time-concentration curve with low coefficient of variance (<0.21) was created to assess pharmacokinetic behaviors. A biexponential curve showed a distribution phase from 1 to 10 min and an elimination phase from 15 to 25 min. The α rate constant was greater than the β rate constant in ROIs, confirming that its pharmacokinetics obeyed a two-compartment model. As a noninvasive technique, combining OMRI imaging with redox probes to monitor tissue red...Continue Reading

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Citations

May 8, 2015·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Fuminori HyodoHideo Utsumi
Mar 12, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Mathieu SarracanieMatthew S Rosen
Nov 5, 2014·Contrast Media & Molecular Imaging·Pierre Danhier, Bernard Gallez
Dec 19, 2012·Medical Hypotheses·Samuel R Cohen, Eric P Cohen
Aug 2, 2016·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Fuminori HyodoHideo Utsumi
Apr 27, 2017·Magnetic Resonance in Chemistry : MRC·D David JebarajA Milton Franklin Benial
Jun 10, 2017·Magnetic Resonance in Chemistry : MRC·V MeenakumariA Milton Franklin Benial
Oct 11, 2017·Antioxidants & Redox Signaling·Shun KishimotoDavid J Lurie
Jul 3, 2013·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hideo Utsumi
Oct 7, 2020·Scientific Reports·Md Zahangir HosainYoshiki Katayama
Dec 23, 2020·Journal of Magnetic Resonance·Ayano Enomoto, Kazuhiro Ichikawa

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