PMID: 7545231Jun 1, 1995Paper

Whole-cell and single-channel currents activated by GABA and glycine in granule cells of the rat cerebellum

The Journal of Physiology
M KanedaS G Cull-Candy

Abstract

1. Patch-clamp methods have been used to characterize GABA-and glycine-activated channels and spontaneous synaptic currents in granule cells in thin cerebellar slices from 7- to 20-day-old rats. 2. All granule cells responded to 10 microM GABA, while approximately 60% responded to 100 microM glycine. With repeated against application, whole-cell responses to GABA, but not those to glycine, declined over a period of minutes unless the pipette solution contained Mg-ATP. 3. Whole-cell concentration-response curves gave EC50 values at 45.2 and 99.6 microM and Hill slopes of 0.94 and 2.6 for GABA and glycine, respectively. At saturating concentrations, currents evoked by GABA were fivefold larger than those evoked by glycine. 4. Whole-cell current-voltage (I-V) relationships of GABA- and glycine-activated currents reversed close to the predicted Cl- equilibrium potential. Partial replacement of intracellular Cl- with F- shifted the GABA reversal potential to a more negative value. 'Instantaneous' I-V relationships produced by ionophoretic application of GABA were linear, while 'steady-state' I-V relationships produced by ramp changes in potential showed outward rectification. For glycine, 'steady-state' I-V plots were linear. 5. Res...Continue Reading

Citations

Jan 1, 1996·Neuropharmacology·W WisdenS Bahn
Dec 1, 2006·The Cerebellum·Jean-Luc DupontJean-Louis Bossu
Oct 15, 2009·The Cerebellum·Marylka Uusisaari, Thomas Knöpfel
May 2, 2012·The Cerebellum·Daniel L KilpatrickE David Litwack
Aug 28, 2004·The Journal of Physiology·Hao HuangAngélique Bordey
Mar 31, 2007·The Journal of Physiology·Stephen H Hadley, Jahanshah Amin
Feb 19, 2002·The Journal of Physiology·Masahiro MoriUrs Gerber
May 26, 2007·The Journal of Physiology·Joseph Glykys, Istvan Mody
Oct 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ian DuguidMichael Häusser
Feb 8, 2005·The Journal of Physiology·Pauline Cavelier, David Attwell
Aug 3, 2014·The Journal of Physiology·Toshiyuki Ishii, Makoto Kaneda
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Oct 19, 2011·Alcoholism, Clinical and Experimental Research·Paolo BottaC Fernando Valenzuela
Sep 18, 2010·The European Journal of Neuroscience·Nadine I HolterAndreas Draguhn
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Jul 14, 2004·European Journal of Pharmacology·Daniele V S LopesFrançois Noël
Sep 17, 2004·Pharmacology & Therapeutics·Stefano Vicini, Pavel Ortinski

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