Whole cell based electrical impedance sensing approach for a rapid nanotoxicity assay

Nanotechnology
Evangelia HondroulisChen-Zhong Li

Abstract

A whole cell based biosensor for rapid real-time testing of human and environmental toxicity of nanoscale materials is reported. Recent studies measuring nanoparticle cytotoxicity in vitro provide a final measurement of toxicity to a cell culture overlooking the ongoing cytotoxic effects of the nanoparticles over the desired timeframe. An array biosensor capable of performing multiple cytotoxicity assays simultaneously was designed to address the need for a consistent method to measure real-time assessments of toxicity. The impedimetric response of human lung fibroblasts (CCL-153) and rainbow trout gill epithelial cells (RTgill-W1) when exposed to gold and silver nanoparticles (AuNPs, AgNPs), single walled carbon nanotubes (SWCNTs) and cadmium oxide (CdO) was tested. Exposure to CdO particles exhibited the fastest rate of cytotoxicity and demonstrated the biosensor's ability to monitor toxicity instantaneously in real time. Advantages of the present method include shorter run times, easier usage, and multi-sample analysis leading to a method that can monitor the kinetic effects of nanoparticle toxicity continuously over a desired timeframe.

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Citations

Sep 17, 2011·Analytical and Bioanalytical Chemistry·Elisa Michelini, Aldo Roda
Jun 25, 2013·Biomedical Microdevices·Evangelia HondroulisChen-Zhong Li
Sep 29, 2011·Applied Biochemistry and Biotechnology·Alicia Fernandez-FernandezAnthony J McGoron
Jun 16, 2012·International Journal of Nanomedicine·Jonathan A CoulterKevin M Prise
Feb 26, 2014·The Analyst·Pratikkumar ShahChen-Zhong Li
Apr 14, 2012·The Analyst·Fareid AsphahaniMiqin Zhang
Nov 14, 2015·Analytical Chemistry·Ian L Gunsolus, Christy L Haynes
Apr 4, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Linda M BrennanWilliam H van der Schalie
May 20, 2011·Biosensors & Bioelectronics·Chen-zhong LiEugenio Almeide
Jul 31, 2013·Journal of Biomedical Materials Research. Part a·Ok Ja YoonNae-Eung Lee
Aug 15, 2013·Molecular Pharmaceutics·Vadym N MochalinYury Gogotsi
Jun 9, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Andrew R CollinsMaria Dusinska
Sep 27, 2016·International Journal of Molecular Sciences·Xi-Feng ZhangSangiliyandi Gurunathan
Aug 7, 2014·Scientific Reports·S Vinjimore KesavanC Allier
May 16, 2020·Frontiers in Bioengineering and Biotechnology·Ravikumar B ShindePandiaraj Manickam
Aug 21, 2013·Biomedical Microdevices·Pratikkumar ShahChen-Zhong Li
Dec 19, 2014·ACS Applied Materials & Interfaces·Anna Regiel-FutyraAgnieszka Kyzioł

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