Whole-cell biocatalysis for selective and productive C-O functional group introduction and modification

Chemical Society Reviews
Manfred SchreweAndreas Schmid


During the last decades, biocatalysis became of increasing importance for chemical and pharmaceutical industries. Regarding regio- and stereospecificity, enzymes have shown to be superior compared to traditional chemical synthesis approaches, especially in C-O functional group chemistry. Catalysts established on a process level are diverse and can be classified along a functional continuum starting with single-step biotransformations using isolated enzymes or microbial strains towards fermentative processes with recombinant microorganisms containing artificial synthetic pathways. The complex organization of respective enzymes combined with aspects such as cofactor dependency and low stability in isolated form often favors the use of whole cells over that of isolated enzymes. Based on an inventory of the large spectrum of biocatalytic C-O functional group chemistry, this review focuses on highlighting the potentials, limitations, and solutions offered by the application of self-regenerating microbial cells as biocatalysts. Different cellular functionalities are discussed in the light of their (possible) contribution to catalyst efficiency. The combined achievements in the areas of protein, genetic, metabolic, and reaction engine...Continue Reading


Jan 1, 1976·International Review of Cytology·P GiesbrechtB Reinicke
Jan 1, 1992·Critical Reviews in Biotechnology·M Mattey
Jan 1, 1990·Methods in Enzymology·P W Trudgill
Mar 1, 1990·Trends in Genetics : TIG·J E McCarthy, C Gualerzi
Jan 1, 1986·Annual Review of Biochemistry·T Platt
May 10, 1974·Annals of the New York Academy of Sciences·L Leive
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A M ChakrabartyI C Gunsalus
Feb 1, 1970·Journal of Bacteriology·J M ShivelyJ W Greenawalt
Feb 5, 1971·Biochemical and Biophysical Research Communications·J M LebeaultM J Coon
Mar 1, 1969·Journal of Bacteriology·E Gantt, S F Conti
Aug 12, 1983·Biochemical and Biophysical Research Communications·J GeigertJ Wadsworth
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·L YuanD J Hawkins
Jan 1, 1995·Annual Review of Microbiology·J W CostertonH M Lappin-Scott
Mar 1, 1996·Trends in Biotechnology·S Y Lee
Oct 29, 1996·Biochimica Et Biophysica Acta·F J Weber, Jan A M de Bont
Feb 14, 1997·The Journal of Biological Chemistry·Juan-Luis RamosA Fernández-Barrero
Nov 15, 1997·Applied and Environmental Microbiology·K E O'ConnorS Hartmans
Feb 3, 1998·Trends in Microbiology·I J van der Klei, M Veenhuis
Oct 23, 1998·Extremophiles : Life Under Extreme Conditions·Andreas SchmidB Witholt
Nov 26, 1998·The Journal of Biological Chemistry·U SchellerW H Schunck
May 20, 1999·Annual Review of Pharmacology and Toxicology·T P DaltonA Puga
May 26, 1999·Applied Microbiology and Biotechnology·D Riesenberg, R Guthke
Jul 9, 1999·Biotechnology and Bioengineering·Steven D DoigD C Stuckey
Sep 1, 1999·Proceedings. Biological Sciences·T Vellai, G Vida
Sep 11, 1999·Trends in Biotechnology·G J Lye, John M Woodley
Dec 23, 1999·Current Opinion in Microbiology·B L Bassler
Jun 1, 2000·Bioscience, Biotechnology, and Biochemistry·T ShibasakiA Ozaki
Sep 1, 2000·Applied Microbiology and Biotechnology·J WeryJan A M de Bont
Oct 29, 2000·Journal of Bacteriology·H KobayashiK Horikoshi
Dec 5, 2000·Current Opinion in Biotechnology·F van Rantwijk, R A Sheldon
Feb 13, 2001·Enzyme and Microbial Technology·S D. DoigJohn M Woodley
Feb 24, 2001·Nature·A SchmidB Witholt
Apr 3, 2001·Current Opinion in Chemical Biology·Jon D Stewart
May 19, 2001·European Journal of Biochemistry·A B Carmichael, L L Wong
May 30, 2001·Journal of Biotechnology·Leonie E HüskenRik Beeftink
Jun 21, 2001·Applied Microbiology and Biotechnology·Leonie E HüskenJ Wery
May 31, 1996·The Journal of Organic Chemistry·Frederick J. Lakner, Lowell P. Hager
Dec 26, 2001·Nature Biotechnology·Stephanie G BurtonJohn M Woodley
Feb 2, 2002·Applied and Environmental Microbiology·Bruno BühlerAndreas Schmid
Apr 17, 2002·Applied Microbiology and Biotechnology·J HaberlandA Liese
May 7, 2002·Advances in Biochemical Engineering/biotechnology·An-Ping Zeng, Hanno Biebl
May 15, 2002·Journal of Biotechnology·Joachim JoseFrank Hannemann
May 29, 2002·Chemistry & Biology·Brenda ValderramaRafael Vazquez-Duhalt
Jun 5, 2002·Journal of Biotechnology·Leonie E HüskenRik Beeftink
Jun 21, 2002·Journal of Industrial Microbiology & Biotechnology·A BerryW Weyler
Jul 27, 2002·Annual Review of Microbiology·Juan L RamosAna Segura
Sep 27, 2002·Current Opinion in Biotechnology·Geoff P Lin CereghinoJames M Cregg
Oct 5, 2002·Biotechnology Progress·Steven D DoigJohn M Woodley
Oct 9, 2002·Nature Biotechnology·Anton GliederFrances H Arnold
Dec 17, 2002·Current Opinion in Biotechnology·Adrie J J StraathofAndreas Schmid
Jan 7, 2003·Nature Biotechnology·Florence Ménard SzczebaraBruno Dumas
Jan 17, 2003·Biotechnology and Bioengineering·Bruno BühlerAndreas Schmid
Apr 8, 2003·Trends in Biotechnology·Jan B van BeilenBernard Witholt
Apr 18, 2003·Biotechnology and Bioengineering·Bruno BühlerAndreas Schmid
May 16, 2003·Advances in Biochemical Engineering/biotechnology·Daniel Stark, Urs von Stockar
Jul 3, 2003·Trends in Biotechnology·Véronique AlphandJohn M Woodley
Jul 3, 2003·Applied Microbiology and Biotechnology·M KataokaS Shimizu
Aug 29, 2003·Current Opinion in Biotechnology·Wilfred A van der Donk, Huimin Zhao
Jun 1, 1961·Journal of Molecular Biology·F JACOB, J MONOD
Oct 29, 2003·Current Opinion in Biotechnology·Charles E Nakamura, Gregory M Whited
Dec 10, 2003·Current Opinion in Biotechnology·Huimin Zhao, Wilfred A van der Donk
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Apr 3, 2004·Biotechnology Progress·Adam Z Walton, Jon D Stewart
Apr 20, 2004·Advances in Biochemical Engineering/biotechnology·Karl Friehs
Jul 13, 2004·Trends in Biotechnology·Claes GustafssonJeremy Minshull
Aug 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·J Kaplan, W F DeGrado
Aug 27, 2004·Biotechnology and Bioengineering·Ye Ni, Rachel Ruizhen Chen
Sep 24, 2004·Journal of Biotechnology·Bruno Bühler, Andreas Schmid
Mar 29, 2005·Advances in Biochemical Engineering/biotechnology·R Wichmann, Durda Vasić-Racki
Jun 1, 2005·Trends in Biotechnology·Sang Yup LeeTae Yong Kim
Jun 18, 2005·Current Opinion in Biotechnology·Jan B van Beilen, Enrico G Funhoff
Jun 18, 2005·Current Opinion in Biotechnology·Ivan MijakovicPeter Ruhdal Jensen
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hal S AlperGregory Stephanopoulos
Sep 13, 2005·Annual Review of Microbiology·Alan G Hinnebusch
Dec 6, 2005·Biotechnology Letters·Ana Vrsalović Presecki, Durda Vasić-Racki
Mar 1, 1984·Applied and Environmental Microbiology·I BartelsW Reineke
Dec 29, 2005·Chembiochem : a European Journal of Chemical Biology·Oliver LentzVlada B Urlacher
Jan 18, 2006·Journal of Biotechnology·Holger PfruenderDirk Weuster-Botz
Jan 18, 2006·The Journal of Biological Chemistry·Heedeok HongBert van den Berg
Jan 21, 2006·Biotechnology and Bioengineering·Jin-Byung Park, Douglas S Clark
Feb 14, 2006·Journal of Industrial Microbiology & Biotechnology·Scott FrykmanPeter Licari
Feb 21, 2006·Trends in Biotechnology·Frank HollmannAndreas Schmid
Mar 7, 2006·Journal of Biotechnology·Rita Bernhardt
Mar 10, 2006·Applied Microbiology and Biotechnology·Thomas A Bobik
Mar 21, 2006·Biochemical Society Transactions·John M Woodley
Jun 13, 2006·Biotechnology and Bioengineering·Jin-Byung ParkAndreas Schmid
Jul 6, 2006·Trends in Biotechnology·Samuel WagnerJan-Willem de Gier
Jul 11, 2006·Advances in Applied Microbiology·Daniel MeyerAndreas Schmid
Jul 14, 2006·Biotechnology and Bioengineering·Patrick C CirinoLonnie O Ingram
Dec 13, 2006·Advances in Applied Microbiology·John M Woodley
Jan 2, 2007·Journal of Biotechnology·Alexander KernAnton Glieder
Jan 16, 2007·Applied Microbiology and Biotechnology·Rachel Ruizhen Chen
Jan 31, 2007·Applied Microbiology and Biotechnology·Hermann J HeipieperFriedhelm Meinhardt
Feb 3, 2007·Biotechnology Progress·Pei-Yi KimJohn M Woodley
Apr 21, 2007·Molecular & Cellular Proteomics : MCP·Samuel WagnerJan-Willem de Gier
May 9, 2007·Applied Microbiology and Biotechnology·Katja GoldbergAndreas Liese
May 16, 2007·Applied Microbiology and Biotechnology·Carsten Bäumchen, Stephanie Bringer-Meyer
Jul 13, 2007·Metabolic Engineering·Xinkai XieYi Tang
Sep 11, 2007·Journal of Biotechnology·Kirsten SchroerStephan Lütz
Jan 15, 2008·Applied and Environmental Microbiology·Bruno BühlerAndreas Schmid
Feb 9, 2008·Current Opinion in Chemical Biology·Mattijs K JulsingAndreas Schmid
Mar 8, 2008·Science·Lin JiangDavid Baker
Mar 18, 2008·Applied and Environmental Microbiology·Rintze M ZelleAntonius J A van Maris
Mar 21, 2008·Nature·Daniela RöthlisbergerDavid Baker
May 15, 2008·Biotechnology and Bioengineering·Jenna L E MorrishAndrew J Daugulis
May 1, 1991·Biotechnology and Bioengineering·E BattatI Goldberg
Jul 16, 2008·Journal of Molecular Biology·Rudi FasanFrances H Arnold
Oct 20, 1996·Biotechnology and Bioengineering·M G WubboltsB Witholt
Sep 25, 2008·Biotechnology and Bioengineering·Stephan LuetzJames Lalonde
Jan 27, 2009·Chemical Society Reviews·Ulf HanefeldEdmond Magner
Mar 20, 2009·Nature·Ronald L KoderP Leslie Dutton
Mar 28, 2009·Journal of the Royal Society, Interface·Mark WelchJeremy Minshull
Sep 18, 2009·PloS One·Mark WelchClaes Gustafsson
Oct 24, 2009·Biochemical and Biophysical Research Communications·Tomohiro KoderaSakayu Shimizu
Oct 30, 2009·Organic & Biomolecular Chemistry·H M de HoogI W C E Arends
Mar 31, 1925·The Journal of Experimental Medicine·E Gorter, F Grendel
Nov 6, 2009·Biotechnology and Bioengineering·José Manuel Otero, Jens Nielsen
Dec 18, 2009·Current Opinion in Chemical Biology·Daniel E Torres PazmiñoMarco W Fraaije
Dec 29, 2009·Current Opinion in Chemical Biology·Fernando Lopez-Gallego, Claudia Schmidt-Dannert
Dec 29, 2009·Journal of Biomedicine & Biotechnology·George H McArthur, Stephen S Fong
Jan 12, 2010·Antioxidants & Redox Signaling·Lars M BlankBruno Bühler
Feb 13, 2010·Trends in Biotechnology·Lucia GardossiEuropean Federation of Biotechnology Section on Applied Biocatalysis
Mar 17, 2010·Biotechnology and Bioengineering·Babu HalanKatja Buehler
Jun 10, 2010·Chembiochem : a European Journal of Chemical Biology·Bert van den Berg
Jul 29, 2010·Applied Microbiology and Biotechnology·Sergey V SmirnovSakayu Shimizu
Sep 10, 2010·Annual Review of Microbiology·Cheryl A KerfeldGordon C Cannon
Sep 15, 2010·Biotechnology Advances·Carla C C R de Carvalho
Oct 5, 2010·Journal of Industrial Microbiology & Biotechnology·F Ozde UtkürAndreas Schmid
Oct 13, 2010·Journal of the American Chemical Society·Wenhua LuRichard A Gross
Nov 17, 2010·Chemical Society Reviews·Jared C LewisFrances H Arnold
May 11, 2011·Molecular Systems Biology·Mary J DunlopAindrila Mukhopadhyay
May 24, 2011·Methods in Enzymology·Keith E J TyoGregory Stephanopoulos
Jul 20, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Reto RuinatschaAndreas Schmid
Jul 26, 2011·Trends in Biotechnology·Vlada B Urlacher, Marco Girhard
Sep 23, 2011·Biotechnology for Biofuels·Mary J Dunlop
Oct 25, 2011·Nucleic Acids Research·Robert J ConradoMatthew P DeLisa
Nov 8, 2011·Current Opinion in Biotechnology·Tae Yong KimSang Yup Lee
Dec 14, 2011·Current Opinion in Biotechnology·Ana SeguraJuan-Luis Ramos
Jan 5, 2012·PloS One·Florent BernaudatNorbert Rolland
Jan 12, 2012·Chemical Society Reviews·Christopher M Clouthier, Joelle N Pelletier
Jan 13, 2012·Current Opinion in Biotechnology·Christopher V Rao
Jan 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Patrick J WestfallChris J Paddon
Jan 24, 2012·Current Opinion in Biotechnology·Amanda M LanzaHal S Alper
Feb 9, 2012·Metabolic Engineering·Jay D Keasling
Mar 20, 2012·Journal of Biotechnology·Stephanie D SchumacherJoachim Jose
Mar 20, 2012·PloS One·Swati ChoudharyClaudia Schmidt-Dannert
Apr 25, 2012·Journal of Industrial Microbiology & Biotechnology·Daniel KuhnAndreas Schmid
Apr 26, 2012·Bioresource Technology·Rui Xue, John M Woodley
May 23, 2012·Archives of Biochemistry and Biophysics·Surabhi Mishra, James Imlay
May 26, 2012·Metabolic Engineering·Vikramaditya G YadavGregory Stephanopoulos
Jun 12, 2012·Applied and Environmental Microbiology·Mattijs K JulsingBruno Bühler
Jun 19, 2012·Trends in Biotechnology·Babu HalanAndreas Schmid
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Robert G Egbert, Eric Klavins
Jun 1, 2010·Environmental Microbiology Reports·Vanina GarcíaAna Segura
Dec 1, 2000·Angewandte Chemie·Michael WolbergMichael Müller


Jun 29, 2013·Chemical Society Reviews·Andreas S Bommarius, Mariétou F Paye
Jan 30, 2016·Journal of the American Chemical Society·Karin M KroneDetlev Belder
Aug 15, 2015·Microbial Biotechnology·Manuel FerrerPeter N Golyshin
Nov 4, 2015·Journal of Biotechnology·Nishanka Dilini RatnayakeKevin D Walker
Feb 6, 2015·Applied Microbiology and Biotechnology·Marie T Lundemo, John M Woodley
Mar 20, 2015·Biotechnology and Bioengineering·Christian WillrodtMattijs K Julsing
Jun 14, 2014·Journal of Biotechnology·Christina A MüllerUlrich Schwaneberg
Apr 4, 2015·Current Opinion in Microbiology·Jan VolmerBruno Bühler
Jul 29, 2015·Microbial Cell Factories·Eleni TheodosiouAndreas Schmid
Apr 29, 2015·Journal of Industrial Microbiology & Biotechnology·Jonathan CollinsBruno Bühler
Apr 17, 2015·Journal of Industrial Microbiology & Biotechnology·Martin LindmeyerAndreas Schmid
Nov 18, 2015·Biotechnology and Bioengineering·Christian WillrodtMattijs K Julsing
Nov 18, 2015·Biotechnology and Bioengineering·Frank W StröhleDirk Holtmann
Feb 19, 2016·Biotechnology and Bioengineering·Florian TievesVlada B Urlacher
Feb 9, 2016·Journal of Biotechnology·Laurence SoussanDelphine Paolucci-Jeanjean
Oct 25, 2016·Journal of Industrial Microbiology & Biotechnology·Christina A MüllerUlrich Schwaneberg
Dec 17, 2016·Scientific Reports·Jonita StankevičiūtėRolandas Meškys
Dec 25, 2016·Journal of Industrial Microbiology & Biotechnology·Marvin KadischBruno Bühler
May 4, 2017·Chemical Reviews·Jonathan LathamJason Micklefield
Oct 29, 2014·Chemical Communications : Chem Comm·Valentin Köhler, Nicholas J Turner
Jun 7, 2014·Chemical Communications : Chem Comm·Dirk HoltmannFrank Hollmann
Aug 11, 2017·Chemical Communications : Chem Comm·Guangyue LiManfred T Reetz
Mar 10, 2018·Applied Microbiology and Biotechnology·Volker F WendischFernando Pérez-García
Feb 18, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Robert JohnstonMichaelann Tartis
Jan 10, 2018·Biophysics Reviews·Ksenia S Egorova, Valentine P Ananikov
May 18, 2018·Chemical Communications : Chem Comm·R A Sheldon, Dean Brady
Jun 21, 2018·Chemical Society Reviews·Joshua BrittonGregory A Weiss
Dec 23, 2014·Chemistry : a European Journal·Bi-Shuang ChenUlf Hanefeld
Jun 13, 2019·Applied Microbiology and Biotechnology·Tianzhen XiongYujie Cai
May 13, 2020·Microbial Biotechnology·Xiulai ChenLiming Liu
Jun 20, 2020·Angewandte Chemie·Shuke WuUwe T Bornscheuer
Oct 6, 2017·Chembiochem : a European Journal of Chemical Biology·Thomas WiesingerFlorian Rudroff
Dec 22, 2017·Applied Microbiology and Biotechnology·Hui LiuBi-Shuang Chen
Jun 15, 2017·Microbial Cell Factories·Baixue Lin, Yong Tao
Sep 12, 2018·World Journal of Microbiology & Biotechnology·Lei Han, Bo Liang
Aug 9, 2020·Scientific Reports·Bjørge WesterengVincent G H Eijsink
Nov 19, 2015·Microbial Cell Factories·Ilka SührerKathrin Castiglione
Aug 29, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hui LiuBi-Shuang Chen
Mar 17, 2020·Frontiers in Bioengineering and Biotechnology·Lisa SchäferBruno Bühler
Feb 13, 2019·Chemical Science·Zhen Zhen ZhouRen Xiang Tan
Jul 19, 2017·Biotechnology Journal·Marvin KadischAndreas Schmid
Apr 13, 2017·Biotechnology and Bioengineering·Jan SchüürmannJoachim Jose
Dec 29, 2020·Microbial Biotechnology·Lisa BretschneiderRohan Karande
Nov 15, 2020·Applied Biochemistry and Biotechnology·Michael D MozuchPhilip J Kersten
Jan 21, 2021·Trends in Biotechnology·Julia JodlbauerFlorian Rudroff

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