Whole-cell current noise produced by excitatory and inhibitory amino acids in large cerebellar neurones of the rat

The Journal of Physiology
S G Cull-Candy, M M Usowicz

Abstract

1. Membrane noise and current changes produced by glutamate and related excitatory amino acids have been examined in cultured large cerebellar neurones (including Purkinje cells), with whole-cell patch-clamp methods. The sensitivity of these neurones to the inhibitory amino acids gamma-aminobutyric acid (GABA) and glycine has also been studied. 2. The neurones formed inhibitory synapses in culture, and displayed spontaneous synaptic currents. Reducing the pipette Cl- concentration (i.e. intracellular synaptic currents. Reducing the pipette Cl- concentration (i.e. intracellular concentration) caused a negative shift in their reversal potential, and the currents could be blocked with bicuculline (10 microM), suggesting that they were mediated by GABAA receptors. Spontaneous synaptic activity was also considerably reduced in the presence of 3 microM-tetrodotoxin. 3. Analysis of the increase in whole-cell current noise produced by the application of GABA (3 microM) gave noise spectra that were fitted by two Lorentzian components with slow and fast time constants of 23.6 and 1.9 ms at a membrane potential (Vm) of -110 mV. The mean single-channel conductance estimated from GABA noise was gamma noise = 12 pS. Glycine (10 microM) whole...Continue Reading

Citations

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