PMID: 9178635Jul 1, 1997Paper

Whole-cell currents from the cloned canine cardiac Na+/Ca2+ exchanger NCX1 overexpressed in a fibroblast cell CCL39

Pflügers Archiv : European journal of physiology
A UeharaI Imanaga

Abstract

A conventional patch-clamp technique was used to record the whole-cell current from the cloned canine cardiac Na+/Ca2+ exchanger NCX1 overexpressed in a fibroblast cell. Ca2+ was extracellularly applied to the Na+-loaded cell to activate the outward current by operating the reverse mode of NCX1. No measurable outward current was ever elicited from the nontransfected cell. Na+/Ca2+ exchange blocker 5 mM Ni2+ or 3 microM KB-R7943 that was applied extracellularly abolished the outward current. With 140 mM external Li+ (replacing Na+), the outward current was transient during the Ca2+ application. In contrast, with 140 mM external Na+, the outward current was maintained without any inactivation during the Ca2+ application. I-V relations predicted from the whole-cell clamp protocols used were obtained both before and during the Ca2+ application. The exchanger whole-cell currents are thus successfully detectable from NCX1 which is overexpressed in this stable transfectant system.

Citations

Aug 4, 2007·Progress in Neurobiology·Tamás L Török
Jun 2, 2007·Journal of Molecular and Cellular Cardiology·Cecilia HurtadoGrant N Pierce

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