Whole-cell membrane currents from human osteoblast-like cells

Calcified Tissue International
C E YellowleyA J Levi

Abstract

Bone cells share common responses to external stimuli with most other cells. Among these are changes in membrane ion channel activity, although at present, relatively little is known about their nature or significance in human bone cells. Using the whole-cell configuration of the patch-clamp technique, we have revealed two types of membrane current in MG63 human osteoblast-like cells. With a potassium-based dialysis solution and a holding potential of -40 mV, voltage commands to more negative potentials elicited an inward current. This current showed little inactivation with time during the command pulse and exhibited some characteristics of an inwardly rectifying K current, including sensitivity to external K and Ba. The second type of current was outward, activated by depolarizing pulses from -40 mV. This current was transient in nature, activating in the first 50 ms of the pulse and then showing rapid inactivation to reach a steady-state level after 4 to 5 seconds. The transient outward current was sensitive to block by TEA, CTX, and to a lesser extent, Ba. These data suggest that a large proportion of this outward current is carried by K ions through channels that may be sensitive to the internal Ca ion concentration. The t...Continue Reading

Citations

Sep 13, 2008·Calcified Tissue International·Koji HirukawaAkifumi Togari
Sep 15, 2012·The Journal of Membrane Biology·Xiantao LiJun Xiao
Jan 11, 2007·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Ronald ZernickeCaeley Lorincz
Sep 25, 2009·American Journal of Physiology. Cell Physiology·Neil C HenneyBronwen A J Evans
Sep 4, 2007·Biosensors & Bioelectronics·Jinjiang YuMo Yang
Jun 13, 2006·Transplant International : Official Journal of the European Society for Organ Transplantation·Dörte KrockerGeorg Matziolis
Oct 27, 2005·Journal of Cellular Physiology·Steven HughesAlicia J El Haj
Jan 6, 2010·Journal of Cellular Physiology·Henrique B SilvaJosé H M Nascimento
Mar 22, 2016·International Journal of Molecular Sciences·Ji Eun YangSo Yeong Lee
Sep 11, 2014·Human Molecular Genetics·Sonia SaccoSaïd Bendahhou

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