PMID: 3746390Jul 1, 1986Paper

Whole-cell voltage-clamp study of the fading of GABA-activated currents in acutely dissociated hippocampal neurons

Journal of Neurophysiology
J R Huguenard, B E Alger

Abstract

The lability of the responses of mammalian central neurons to gamma-aminobutyric acid (GABA) was studied using neurons acutely dissociated from the CA1 region of the adult guinea pig hippocampus as a model system. GABA was applied to the neuronal somata by pressure ejection and the resulting current (IGABA) recorded under whole-cell voltage clamp. In initial experiments we examined several basic properties of cells in this preparation. Our data confirm that passive and active membrane properties are similar to those which characterize cells in other preparations. In addition, GABA-dependent conductance (gGABA), reversal potential (EGABA), and the interaction of GABA with pentobarbital and bicuculline all appeared to be normal. Dendritic GABA application could cause depolarizing GABA responses, and somatic GABA application caused hyperpolarizations due to chloride (Cl-) movements. Repetitive brief applications (5-15 ms) of GABA (10(-5) to 10(-3) M) at a frequency of 0.5 Hz led to fading of successive peaks of IGABA until, at a given holding potential, a steady state was reached in which IGABA no longer changed. Imposing voltage steps lasting seconds during a train of steady-state GABA responses led initially to increased IGABA t...Continue Reading

Citations

Sep 1, 1991·British Journal of Pharmacology·U KristiansenP Krogsgaard-Larsen
Jan 1, 1991·Annals of the New York Academy of Sciences·B E Alger
Jul 1, 1990·Pflügers Archiv : European journal of physiology·N Ogata, H Tatebayashi
Apr 18, 1998·Journal of Neurophysiology·B D BennettD A Prince
Jul 21, 2000·European Journal of Biochemistry·D P Nolan, H P Voorheis
Mar 30, 2012·Journal of Neurophysiology·Liu D LiuWilliam D Hutchison
Aug 18, 1995·Science·N Lambert, L Grover
Mar 19, 2005·Journal of Neurophysiology·Xiaoming JinDavid A Prince
Nov 16, 2011·The Journal of General Physiology·Urban KarlssonStaffan Johansson
Mar 31, 2012·Advances in Pharmacological Sciences·Andrew N Clarkson
Dec 14, 2017·Brain : a Journal of Neurology·Luka MilosevicWilliam D Hutchison
Jul 11, 1998·Journal of Neurophysiology·H L GasparyG B Richerson
Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·F Le FollL Cazin
Feb 5, 2002·Annual Review of Physiology·Eric Delpire, David B Mount
Jun 26, 1998·British Journal of Pharmacology·A LückeE J Speckmann
Jun 1, 1990·British Journal of Pharmacology·T G Allen, G Burnstock
Mar 1, 1991·Neurochemical Research·J G Tasker, F E Dudek
Aug 16, 2011·Epilepsia·Edward H MaaKevin J Staley
Nov 2, 2012·Journal of Neurophysiology·Christopher B RansomGeorge B Richerson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.