Whole-Chain Tick Saliva Proteins Presented on Hepatitis B Virus Capsid-Like Particles Induce High-Titered Antibodies with Neutralizing Potential

PloS One
Philipp KolbMichael Nassal

Abstract

Ticks are vectors for various, including pathogenic, microbes. Tick saliva contains multiple anti-host defense factors that enable ticks their bloodmeals yet also facilitate microbe transmission. Lyme disease-causing borreliae profit specifically from the broadly conserved tick histamine release factor (tHRF), and from cysteine-rich glycoproteins represented by Salp15 from Ixodes scapularis and Iric-1 from Ixodes ricinus ticks which they recruit to their outer surface protein C (OspC). Hence these tick proteins are attractive targets for anti-tick vaccines that simultaneously impair borrelia transmission. Main obstacles are the tick proteins´ immunosuppressive activities, and for Salp15 orthologs, the lack of efficient recombinant expression systems. Here, we exploited the immune-enhancing properties of hepatitis B virus core protein (HBc) derived capsid-like particles (CLPs) to generate, in E. coli, nanoparticulate vaccines presenting tHRF and, as surrogates for the barely soluble wild-type proteins, cysteine-free Salp15 and Iric-1 variants. The latter CLPs were exclusively accessible in the less sterically constrained SplitCore system. Mice immunized with tHRF CLPs mounted a strong anti-tHRF antibody response. CLPs presenting...Continue Reading

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Citations

Aug 23, 2012·PloS One·Maria TagliamonteLuigi Buonaguro
Jan 1, 2019·Biotechnology and Bioengineering·Hayley K Charlton HumeLinda H L Lua
Sep 12, 2020·Biomaterials Science·Sourabh ShuklaNicole F Steinmetz
Jan 1, 2018·Biologie aujourd'hui·Nathalie Boulanger
Jan 31, 2020·Frontiers in Immunology·Shiyuan WenFukai Bao
Apr 17, 2019·Bioconjugate Chemistry·Jasmin FederizonJonathan F Lovell

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Datasets Mentioned

BETA
AFA85556.1
and
AIK19145.1

Methods Mentioned

BETA
X-ray
PCR
ELISA
size exclusion chromatography
ion exchange chromatography
electrophoresis
electron microscopy
ELISAs
Assay
glycosylation

Software Mentioned

Graphpad Prism
GeneArt
HBc CLP
SplitCore

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