PMID: 9419749Jan 1, 1994Paper

Whole endometrial fragments form characteristics of in vivo endometriosis in a mesothelial cell co-culture system: an in vitro model for the study of the histogenesis of endometriosis

Journal of the Society for Gynecologic Investigation
R A WildD Medders

Abstract

We have previously reported on the effects of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) on the adhesion of human endometrial stromal cells to peritoneal mesothelial cells in vitro. The relevance of this co-culture system to in vivo endometriosis remains to be established. We contrasted endometrial fragments with endometrial stromal cells to determine their relevance. Human mesothelial cells were isolated from peritoneal fluid from four normal women via Ficoll-Paque gradient centrifugation at 400 x g for 30 minutes and grown in M199 medium containing epidermal growth factor (10 ng/mL) and 10% fetal calf serum. The homogeneous cells were cultured on collagen-coated plates until a monolayer formed. Endometrial stromal cells or whole endometrial fragments, isolated from endometrial tissue of four normal women, were put on the mesothelial monolayer and cultured at 37 degrees C for 24 days. After fixation, the samples were incubated with monoclonal antibody to epithelial membrane antigen, cytokeratin, and vimentin, and processed with standard immunohistochemical techniques. Observation under phase contrast microscopy revealed that, in this co-culture system, whole endometrial fragments demonstrated characterist...Continue Reading

References

Apr 1, 1979·The Journal of Clinical Endocrinology and Metabolism·P G SatyaswaroopE Gurpide

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Citations

Jun 24, 2009·Reproductive Sciences·Jason S GriffithRobert S Schenken
Apr 13, 2002·Annals of the New York Academy of Sciences·Craig A WitzRobert S Schenken
Feb 20, 2015·Biology of Reproduction·Jenny N FungGrant W Montgomery
Feb 28, 2020·Experimental and Therapeutic Medicine·Hongjie Fan

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