Whole-Exome Enrichment with the Agilent SureSelect Human All Exon Platform

Cold Spring Harbor Protocols
Rui ChenMichael Snyder

Abstract

There are multiple platforms available for whole-exome enrichment and sequencing (WES). This protocol is based on the Agilent SureSelect Human All Exon platform, which targets ∼50 Mb of the human exonic regions. The SureSelect system uses ∼120-base RNA probes to capture known coding DNA sequences (CDS) from the NCBI Consensus CDS Database as well as other major RNA coding sequence databases, such as Sanger miRBase. The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.

References

Sep 29, 2011·Nature Biotechnology·Michael J ClarkMichael Snyder
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder

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Citations

Dec 19, 2015·Expert Review of Molecular Diagnostics·Leomar Y BallesterRajesh R Singh
May 3, 2018·Molecular Biology and Evolution·Atsushi NiidaHideki Innan
Jan 13, 2018·Frontiers in Genetics·Amina KamarFadi Bitar
Oct 27, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Chunze ZhangXichuan Li
Mar 2, 2018·Frontiers in Genetics·Arlene J GeorgeAngela M Mabb
Nov 6, 2018·Scientific Reports·Muhammad Jaseem KhanAlexander N Yatsenko
Dec 14, 2018·Frontiers in Microbiology·Maxime Gaudin, Christelle Desnues
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder

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