Whole-Exome Enrichment with the Illumina TruSeq Exome Enrichment Platform

Cold Spring Harbor Protocols
Rui ChenMichael Snyder

Abstract

Multiple platforms are available for whole-exome enrichment and sequencing (WES). This protocol is based on the Illumina TruSeq Exome Enrichment platform, which captures ∼62 Mb of the human exonic regions using 95-base DNA probes. In addition to covering the RefSeq and Ensembl coding sequences, the enriched sequences also include ∼28 Mb of RefSeq untranslated regions (UTR). The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.

References

Sep 29, 2011·Nature Biotechnology·Michael J ClarkMichael Snyder
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder

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Citations

Dec 19, 2015·Expert Review of Molecular Diagnostics·Leomar Y BallesterRajesh R Singh
Oct 17, 2015·The British Journal of Ophthalmology·Huan HuQingjiong Zhang
Dec 14, 2018·Frontiers in Microbiology·Maxime Gaudin, Christelle Desnues
Nov 17, 2020·Frontiers in Genetics·Aquillah M KanziTulio de Oliveira
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder

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