Whole-Exome Enrichment with the Roche NimbleGen SeqCap EZ Exome Library SR Platform

Cold Spring Harbor Protocols
Rui ChenMichael Snyder

Abstract

Multiple platforms are available for whole-exome enrichment and sequencing (WES). This protocol is based on the Roche NimbleGen SeqCap EZ Exome Library SR platform, which enriches for ∼44 Mb of the human exonic regions. The SeqCap system uses 55- to 105-base DNA probes to capture known coding DNA sequences (CDS) from the NCBI Consensus CDS Database, RefSeq, and Sanger miRBase. The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.

References

Sep 29, 2011·Nature Biotechnology·Michael J ClarkMichael Snyder
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder

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Citations

Dec 19, 2015·Expert Review of Molecular Diagnostics·Leomar Y BallesterRajesh R Singh
Apr 17, 2015·BMC Bioinformatics·Sandeep K KushwahaDag Ahrén
Mar 6, 2017·Molecular Medicine Reports·Guoping SunYong Dai
Apr 9, 2017·Familial Cancer·Tremika Le-Shan WilsonTobias Else
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder
Mar 13, 2015·Cold Spring Harbor Protocols·Rui ChenMichael Snyder

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