Whole-exome sequencing in osteosarcoma reveals important heterogeneity of genetic alterations

Annals of Oncology : Official Journal of the European Society for Medical Oncology
M BousquetA Gomez-Brouchet

Abstract

Whole-genome sequencing studies have recently shown that osteosarcomas (OSs) display high rates of structural variation, i.e. they contain many somatic mutations and copy number alterations. TP53 and RB1 show recurrent somatic alterations in concordant studies, suggesting that they could be key players in bone oncogenesis. we carried out whole-genome sequencing of DNA from seven high-grade OS samples matched with normal tissue from the same patients. We confirmed the presence of genetic alterations of the TP53 (including novel unreported mutations) and RB1 genes. Most interestingly, we identified a total of 84 point mutations and 4 deletions related to 82 different genes in OS samples, of which only 15 have been previously reported. Interestingly, the number of mutated genes (ranging from 4 to 8) was lower in TP53mut cases compared with TP53wt cases (ranging from 14 to 45). This was also true for the mutated RB1 case. We also observed that a dedifferentiated OS harboring MDM2 amplification did not carry any other mutations. This study suggests that bone oncogenesis driven by TP53 or RB1 mutations occurs on a background of relative genetic stability and that the dedifferentiated OS subtype represents a clinico-pathological entit...Continue Reading

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Oct 21, 2016·Expert Opinion on Investigational Drugs·Marie-Françoise HeymannDominique Heymann
Jan 23, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Fan TangZhenfeng Duan
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