Whole-Genome Analysis Illustrates Global Clonal Population Structure of the Ubiquitous Dermatophyte Pathogen Trichophyton rubrum

Genetics
Gabriela F PersinotiChristina A Cuomo

Abstract

Dermatophytes include fungal species that infect humans, as well as those that also infect other animals or only grow in the environment. The dermatophyte species Trichophyton rubrum is a frequent cause of skin infection in immunocompetent individuals. While members of the T. rubrum species complex have been further categorized based on various morphologies, their population structure and ability to undergo sexual reproduction are not well understood. In this study, we analyze a large set of T. rubrum and T. interdigitale isolates to examine mating types, evidence of mating, and genetic variation. We find that nearly all isolates of T. rubrum are of a single mating type, and that incubation with T. rubrum "morphotype" megninii isolates of the other mating type failed to induce sexual development. While the region around the mating type locus is characterized by a higher frequency of SNPs compared to other genomic regions, we find that the population is remarkably clonal, with highly conserved gene content, low levels of variation, and little evidence of recombination. These results support a model of recent transition to asexual growth when this species specialized to growth on human hosts.

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Nov 22, 2018·Medical Mycology·Ivan M PchelinAnastasia E Taraskina
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Apr 10, 2021·Mycopathologia·Antonio RossiNilce M Martinez-Rossi
Nov 4, 2021·Microbial Genomics·Luc CornetPierre Becker

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Datasets Mentioned

BETA
MPY08200
118892
ACPI00000000
AF170456
CBS118892
PRJNA186851

Methods Mentioned

BETA
Protein
Precipitation
PCR
light microscopy

Software Mentioned

Picard
IndelRealigner GATK
GeneMark
tRNAscan
OrthoMCL
ALLPATHS
HMMER3
BLAST
BaseRecalibrator
RNAmmer

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