Whole-Genome Analysis of an Extensively Drug-Resistant Acinetobacter baumannii Strain XDR-BJ83: Insights into the Mechanisms of Resistance of an ST368 Strain from a Tertiary Care Hospital in China

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Yang-Yang ZhangLiang-An Chen

Abstract

Acinetobacter baumannii is an important pathogen of nosocomial infections. Nosocomial outbreaks caused by antibiotic-resistant A. baumannii remain a significant challenge. Understanding the antibiotic resistance mechanism of A. baumannii is critical for clinical treatment. The purpose of this study was to determine the whole-genome sequence (WGS) of an extensively drug-resistant (XDR) A. baumannii strain, XDR-BJ83, which was associated with a nosocomial outbreak in a tertiary care hospital of China, and to investigate the antibiotic resistance mechanism of this strain. The WGS of XDR-BJ83 was performed using single-molecule real-time sequencing. The complete genome of XDR-BJ83 consisted of a 4,011,552-bp chromosome and a 69,069-bp plasmid. The sequence type of XDR-BJ83 was ST368, which belongs to clonal complex 92 (CC92). The chromosome of XDR-BJ83 carried multiple antibiotic resistance genes, antibiotic efflux pump genes, and mobile genetic elements, including insertion sequences, transposons, integrons, and resistance islands. The plasmid of XDR-BJ83 (pBJ83) was a conjugative plasmid carrying type IV secretion system. These results indicate that the presence of multiple antibiotic resistance genes, efflux pumps, and mobile ge...Continue Reading

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Citations

Apr 17, 2019·Journal of Medical Microbiology·Megha KaushikPooja Gulati
Feb 23, 2019·Frontiers in Microbiology·Jetsi Mancilla-RojanoAriadnna Cruz-Córdova

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Datasets Mentioned

BETA
BJAB07104
BJAB0715
CP018421
CP018422
AB307-0294

Methods Mentioned

BETA
electrophoresis
phosphotransferase

Software Mentioned

Genomic Island Prediction ( GIPSy )
RNAmmer
tRNAscan
SMRT Analysis pipeline
ClustalW
GeneMarkS +
ResFinder
BLAST
SE
Rfam

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