Whole-genome analysis of pseudorabies virus gene expression by real-time quantitative RT-PCR assay

BMC Genomics
Dóra TombáczZsolt Boldogköi

Abstract

Pseudorabies virus (PRV), a neurotropic herpesvirus of pigs, serves as an excellent model system with which to investigate the herpesvirus life cycle both in cultured cells and in vivo. Real-time RT-PCR is a very sensitive, accurate and reproducible technique that can be used to detect very small amounts of RNA molecules, and it can therefore be applied for analysis of the expression of herpesvirus genes from the very early period of infection. In this study, we have developed and applied a quantitative reverse transcriptase-based real-time PCR technique in order to profile transcription from the whole genome of PRV after lytic infection in porcine kidney cells. We calculated the relative expression ratios in a novel way, which allowed us to compare different PRV genes with respect to their expression dynamics, and to divide the PRV genes into distinct kinetic classes. This is the first publication on the whole-genome analysis of the gene expression of an alpha-herpesvirus by qRT2-PCR. We additionally established the kinetic properties of uncharacterized PRV genes and revised or confirmed data on PRV genes earlier examined by traditional methods such as Northern blot analysis. Our investigations revealed that genes with the sam...Continue Reading

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Citations

Feb 8, 2013·BMC Genomics·Steven J van BeurdenMarc Y Engelsma
Dec 8, 2010·BMC Microbiology·Judit S TóthZsolt Boldogkoi
Jun 23, 2011·Future Virology·Emily EshlemanRandall J Cohrs
Apr 1, 2014·Journal of Invertebrate Pathology·Amélie SegarraTristan Renault
May 27, 2015·Viruses·Dóra TombáczZsolt Boldogköi
Jul 2, 2015·BMC Microbiology·Péter OláhZsolt Boldogkoi
Aug 2, 2018·The Journal of General Virology·Imane BoumartCatherine Dupuy
Feb 5, 2016·Journal of Virology·A CriddleM P Taylor
Jan 17, 2020·Journal of Virology·Julia SehlThomas C Mettenleiter
May 17, 2019·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Zsolt CsabaiZsolt Boldogköi
Feb 7, 2018·Frontiers in Microbiology·Norbert MoldovánZsolt Boldogköi
Oct 15, 2019·Frontiers in Genetics·Dóra TombáczZsolt Boldogköi

Methods Mentioned

BETA
electrophoresis
PCR
chip
in

Related Concepts

DNA Replication
Genes, Reiterated
Phosphonoacetate
Herpesvirus 1, Suid
Protein Biosynthesis
Gene Expression Regulation, Viral
Viral Genome
Reverse Transcriptase Polymerase Chain Reaction
MRNA Differential Display
Genomics

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