Whole genome and transcriptome sequencing of matched primary and peritoneal metastatic gastric carcinoma

Scientific Reports
J ZhangY Y Yu

Abstract

Gastric cancer is one of the most aggressive cancers and is the second leading cause of cancer death worldwide. Approximately 40% of global gastric cancer cases occur in China, with peritoneal metastasis being the prevalent form of recurrence and metastasis in advanced disease. Currently, there are limited clinical approaches for predicting and treatment of peritoneal metastasis, resulting in a 6-month average survival time. By comprehensive genome analysis will uncover the pathogenesis of peritoneal metastasis. Here we describe a comprehensive whole-genome and transcriptome sequencing analysis of one advanced gastric cancer case, including non-cancerous mucosa, primary cancer and matched peritoneal metastatic cancer. The peripheral blood is used as normal control. We identified 27 mutated genes, of which 19 genes are reported in COSMIC database (ZNF208, CRNN, ATXN3, DCTN1, RP1L1, PRB4, PRB1, MUC4, HS6ST3, MUC17, JAM2, ITGAD, IREB2, IQUB, CORO1B, CCDC121, AKAP2, ACAN and ACADL), and eight genes have not previously been described in gastric cancer (CCDC178, ARMC4, TUBB6, PLIN4, PKLR, PDZD2, DMBT1and DAB1).Additionally,GPX4 and MPND in 19q13.3-13.4 region, is characterized as a novel fusion-gene. This study disclosed novel biolog...Continue Reading

References

Aug 29, 2003·The British Journal of Surgery·F RovielloUNKNOWN Italian Research Group for Gastric Cancer
Nov 16, 2006·International Journal of Rehabilitation Research. Internationale Zeitschrift Für Rehabilitationsforschung. Revue Internationale De Recherches De Réadaptation·Supalak KhemthongSatvinder S Dhaliwal
Mar 18, 2009·Biochimica Et Biophysica Acta·Regina Brigelius-Flohé, Anna Kipp
Sep 5, 2009·Journal of Cancer Research and Clinical Oncology·Dan HuangXiang Du
Dec 17, 2010·World Journal of Gastrointestinal Oncology·Ying-Yan Yu, Zheng-Gang Zhu
Nov 23, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nataliya Rohr-UdilovaMarkus Peck-Radosavljevic
Mar 23, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marisa MarianiCristiano Ferlini
Aug 21, 2012·Virchows Archiv : an International Journal of Pathology·Cathelijn J F WaaijerJudith V M G Bovée
Sep 1, 2012·Anti-cancer Agents in Medicinal Chemistry·Lei LiuYing Y Yu
Dec 15, 2012·Genome Biology·Niranjan NagarajanYijun Ruan
Mar 13, 2013·Expert Review of Molecular Diagnostics·Hitchintan KaurRaymond R Mattingly
May 21, 2013·Gastroenterology·Alexandra E GylfeLauri A Aaltonen
Jun 7, 2013·Cancer Research·Rachael Natrajan, Paul Wilkerson
Jul 9, 2013·International Journal of Cancer. Journal International Du Cancer·Irene ThomassenIgnace H de Hingh
Feb 28, 2014·Asia-Pacific Journal of Clinical Oncology·Heba Moussa, Iman Sidhom
Aug 1, 2014·Nature·UNKNOWN Cancer Genome Atlas Research Network
Sep 17, 2014·Molecular Cancer Research : MCR·Erica di MartinoMargaret A Knowles
Jan 16, 2015·Epigenomics·Yingying CuiMingzhou Guo

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Citations

Jan 26, 2018·Journal of Cardiovascular Development and Disease·Santosh V SuryavanshiBradley K McConnell
Jul 16, 2016·Biomedit︠s︡inskai︠a︡ khimii︠a︡·Y S KimA Yu Lupatov
Jul 26, 2019·Cell Biochemistry and Function·Yaoyao GuanOuchen Wang
Jan 7, 2016·BMC Medical Informatics and Decision Making·Scott D ConstableSteve Chapin
Jan 4, 2020·Oncology Letters·Jiangang SunYongshun Gao
Apr 29, 2016·Oncology Reports·Huishan ZhaoWen G Jiang
Aug 23, 2018·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Han Hong LeeTae-Min Kim
May 7, 2020·Journal of the Egyptian National Cancer Institute·Dina M El-GuindyAsmaa M Elkady
Sep 11, 2020·Computational and Mathematical Methods in Medicine·Changyuan MengTong Zhou
Feb 27, 2021·Journal of Integrative Plant Biology·Xuehai ZhengTing Xue
Sep 21, 2021·Frontiers in Oncology·Bingdong ZhangYongbing Chen

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Methods Mentioned

BETA
surgical resection
RNA-seq
PCR
Illumina Sequencing

Software Mentioned

Picard
IndelRealigner
BWA
TopHat
GATK Unified Genotyper
Samtools
Cufflinks
GATK

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