Whole-genome assembly of Babesia ovata and comparative genomics between closely related pathogens

BMC Genomics
Junya YamagishiShin-Ichiro Kawazu

Abstract

Babesia ovata, belonging to the phylum Apicomplexa, is an infectious parasite of bovids. It is not associated with the manifestation of severe symptoms, in contrast to other types of bovine babesiosis caused by B. bovis and B. bigemina; however, upon co-infection with Theileria orientalis, it occasionally induces exacerbated symptoms. Asymptomatic chronic infection in bovines is usually observed only for B. ovata. Comparative genomic analysis could potentially reveal factors involved in these distinguishing characteristics; however, the genomic and molecular basis of these phenotypes remains elusive, especially in B. ovata. From a technical perspective, the current development of a very long read sequencer, MinION, will facilitate the obtainment of highly integrated genome sequences. Therefore, we applied next-generation sequencing to acquire a high-quality genome of the parasite, which provides fundamental information for understanding apicomplexans. The genome was assembled into 14,453,397 bp in size with 5031 protein-coding sequences (91 contigs and N50 = 2,090,503 bp). Gene family analysis revealed that ves1 alpha and beta, which belong to multigene families in B. bovis, were absent from B. ovata, the same as in B. bigemina...Continue Reading

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Citations

Aug 20, 2019·PLoS Neglected Tropical Diseases·Luis Miguel GonzálezEstrella Montero
May 30, 2019·Parasites & Vectors·Gamila A R BohaligaMassaro W Ueti
Jun 14, 2019·MSphere·Hassan HakimiMasahito Asada
Dec 5, 2020·Molecular Microbiology·Brendan Elsworth, Manoj T Duraisingh
Dec 29, 2020·Genomics·Boitumelo B MabokoBen J Mans
Jan 13, 2021·Pathogens·Monica Florin-ChristensenLeonhard Schnittger
Feb 22, 2021·International Journal for Parasitology·Romina GallentiLeonhard Schnittger
Sep 7, 2021·Frontiers in Cellular and Infection Microbiology·Jinming WangGuiquan Guan

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Datasets Mentioned

BETA
PRJEB5046

Methods Mentioned

BETA
RNAseq

Software Mentioned

SAMmap
Gephi
SAMtools
Tophat2
script
bam2hints
MinION
BLASTN
webAugustus
Canu

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