Whole-genome mapping of 5' RNA ends in bacteria by tagged sequencing: a comprehensive view in Enterococcus faecalis

RNA
Nicolas InnocentiErik Aurell

Abstract

Enterococcus faecalis is the third cause of nosocomial infections. To obtain the first snapshot of transcriptional organizations in this bacterium, we used a modified RNA-seq approach enabling to discriminate primary from processed 5' RNA ends. We also validated our approach by confirming known features in Escherichia coli. We mapped 559 transcription start sites (TSSs) and 352 processing sites (PSSs) in E. faecalis. A blind motif search retrieved canonical features of SigA- and SigN-dependent promoters preceding transcription start sites mapped. We discovered 85 novel putative regulatory RNAs, small- and antisense RNAs, and 72 transcriptional antisense organizations. Presented data constitute a significant insight into bacterial RNA landscapes and a step toward the inference of regulatory processes at transcriptional and post-transcriptional levels in a comprehensive manner.

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Citations

Jan 23, 2016·Frontiers in Microbiology·Sofía Ruiz-CruzAlicia Bravo
Jun 24, 2017·ELife·Karthik HullahalliKelli L Palmer
Sep 6, 2017·PLoS Biology·Marina LizioGuojun Sheng
Apr 25, 2018·Clinical Chemistry·Feng LiDavid T W Wong
Oct 11, 2019·Archives of Microbiology·Marine SalzeCécile Muller
Apr 5, 2016·Physical Biology·Erik AurellHai-Jun Zhou
Apr 21, 2017·Nucleic Acids Research·Alexandre MaesEliane Hajnsdorf
Jan 13, 2019·Viruses·Julien LossouarnMarie-Agnès Petit
Jan 23, 2020·Journal of Bacteriology·Tomoya KozakaiTohru Suzuki
Dec 17, 2020·Frontiers in Cellular and Infection Microbiology·Charlotte MichauxJörg Vogel
Feb 19, 2020·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Emma DesgrangesDavid Lalaouna
Mar 9, 2019·Microbiology Spectrum·Keith E Weaver

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