Whole-Genome Methylation Analysis Reveals Epigenetic Variation in Cloned and Donor Pigs

Frontiers in Genetics
Mengfen WangXiangdong Liu

Abstract

Somatic cloning has had a significant impact on the life sciences and is important in a variety of processes, including medical research and animal production. However, the application of somatic cloning has been limited due to its low success rate. Therefore, potential epigenetic variations between cloned and donor animals are still unclear. DNA methylation, one of the factors which is responsible for phenotypic differences in animals, is a commonly researched topic in epigenetic studies of mammals. To investigate the epigenetic variations between cloned and donor animals, we selected blood and ear fibroblasts of a donor pig and a cloned pig to perform whole-genome bisulfite sequencing (WGBS). A total of 215 and 707 differential methylation genes (DMGs) were identified in blood and ear fibroblasts, respectively. Functional annotation revealed that DMGs are enriched in many pathways, including T/B or natural killer (NK) cell differentiation, oocyte maturation, embryonic development, and reproductive hormone secretion. Moreover, 22 DMGs in the blood and 75 in the ear were associated with immune responses (e.g., CD244, CDK6, CD5, CD2, CD83, and CDC7). We also found that 18 DMGs in blood and 53 in ear fibroblasts were involved in ...Continue Reading

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Citations

Mar 13, 2021·Frontiers in Genetics·Mengqi Wang, Eveline M Ibeagha-Awemu

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Datasets Mentioned

BETA
SUB5307644

Methods Mentioned

BETA
WGBS
PCR

Software Mentioned

Enrich
Illumina Casava1
MOABS
topGO
FastQC
adjust
Bismark
Bowtie2
R
ChIPseeker

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