Whole-Genome Sequence Analysis of CTX-M Containing Escherichia coli Isolates from Retail Meats and Cattle in the United States

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Daniel A TadesseS Zhao

Abstract

In recent years, there have been increased reports on the detection of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Salmonella strains from food-producing animals and animal products in the United States. We characterized 18 ESBL E. coli isolates from cattle (n = 5), chicken breast (n = 5), ground turkey (n = 6), ground beef (n = 1), and pork chops (n = 1) that were collected by the National Antimicrobial Resistance Monitoring System (NARMS) between 2011 and 2015. In vitro antimicrobial susceptibility testing was done against a panel of 14 antimicrobials followed by a secondary panel of 9 β-lactam agents. Whole-genome sequencing was used to characterize the resistome, plasmids, and the genetic structures of the ESBL genes. All ESBL-producing E. coli isolates were resistant to at least three antimicrobial classes and carried various blaCTX-M genes. Most of the cattle and ground turkey isolates carried blaCTX-M-27. In chicken breast isolates, blaCTX-M-1 was present as part of an ISEcp1 transposition unit carried on a plasmid that shares sequence similarity with the backbone structure of the IncI plasmid. Isolates carrying the blaCTX-M-14 and blaCTX-M-15 genes, widely distributed in human clinical isolate...Continue Reading

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Citations

Sep 17, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Larissa Alvarenga Batista BotelhoRaquel Regina Bonelli
Jun 4, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Kazal K GhoshJoseph E Rubin
Jul 23, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mariem SaidaniMarisa Haenni
Jul 31, 2021·Frontiers in Cellular and Infection Microbiology·Hazem RamadanCharlene R Jackson

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Sensititre
ResFinder
BLASTX
MiSeq Desktop
BLASTX PERL scripts
PubMLST
PlasmidFinder
SNPhylo
CLC Genomics Workbench

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