Whole Genome Sequence of the Parasitoid Wasp Microplitis demolitor That Harbors an Endogenous Virus Mutualist

G3 : Genes - Genomes - Genetics
Gaelen R BurkeMichael R Strand

Abstract

Microplitis demolitor (Hymenoptera: Braconidae) is a parasitoid used as a biological control agent to control larval-stage Lepidoptera and serves as a model for studying the function and evolution of symbiotic viruses in the genus Bracovirus Here we present the M. demolitor genome (assembly version 2.0), with a genome size of 241 Mb, and a N50 scaffold and contig size of 1.1 Mb and 14 Kb, respectively. Using RNA-Seq data and manual annotation of genes of viral origin, we produced a high-quality gene set that includes 18,586 eukaryotic and 171 virus-derived protein-coding genes. Bracoviruses are dsDNA viruses with unusual genome architecture, in which the viral genome is integrated into the wasp genome and is comprised of two distinct components: proviral segments that are amplified, circularized, and packaged into virions for export into the wasp's host via oviposition; and replication genes. This genome assembly revealed that at least two scaffolds contain both nudivirus-like genes and proviral segments, demonstrating that at least some of these components are near each other in the genome on a single chromosome. The updated assembly and annotation are available in several publicly accessible databases; including the National ...Continue Reading

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Citations

Jan 24, 2021·Communications Biology·Jérémy GauthierJean-Michel Drezen
Feb 17, 2021·The Journal of General Virology·Michael J ArvinMichael R Strand
May 23, 2019·Current Opinion in Insect Science·Gaelen R Burke
May 28, 2021·The Journal of Heredity·Brendan J PintoJennifer M Zaspel

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Datasets Mentioned

BETA
SRX981480

Methods Mentioned

BETA
RNASeq
flow cytometry
RNA-Seq

Software Mentioned

GapCloser
hisat2
gff3
i5k
BUSCO
demolitor
AgDataCommons
fastx
Apollo
samtools

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