Whole-Genome Sequences of Five Acinetobacter baumannii Strains From a Child With Leukemia M2

Frontiers in Microbiology
Jetsi Mancilla-RojanoAriadnna Cruz-Córdova

Abstract

Acinetobacter baumannii is an opportunistic pathogen and is one of the primary etiological agents of healthcare-associated infections (HAIs). A. baumannii infections are difficult to treat due to the intrinsic and acquired antibiotic resistance of strains of this bacterium, which frequently limits therapeutic options. In this study, five A. baumannii strains (810CP, 433H, 434H, 483H, and A-2), all of which were isolated from a child with leukemia M2, were characterized through antibiotic susceptibility profiling, the detection of genes encoding carbapenem hydrolyzing oxacillinases, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), adherence and invasion assays toward the A549 cell line, and the whole-genome sequence (WGS). The five strains showed Multidrug resistant (MDR) profiles and amplification of the blaOXA-23 gene, belonging to ST758 and grouped into two PFGE clusters. WGS of 810CP revealed the presence of a circular chromosome and two small plasmids, pAba810CPa and pAba810CPb. Both plasmids carried genes encoding the Sp1TA system, although resistance genes were not identified. A gene-by-gene comparison analysis was performed among the A. baumannii strains isolated in this study and others A. bau...Continue Reading

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Datasets Mentioned

BETA
CP027611.1
AF401
AB030

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

ISfinder
Prokka
PHAST
GenVision
eBURST
MLST
- genomecov
PhiPack
BLASTn
NTSYS

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