Whole Genome-Sequencing and Phylogenetic Analysis of a Historical Collection of Bacillus anthracis Strains from Danish Cattle

PloS One
Sylviane DerzelleØystein Angen

Abstract

Bacillus anthracis, the causative agent of anthrax, is known as one of the most genetically monomorphic species. Canonical single-nucleotide polymorphism (SNP) typing and whole-genome sequencing were used to investigate the molecular diversity of eleven B. anthracis strains isolated from cattle in Denmark between 1935 and 1988. Danish strains were assigned into five canSNP groups or lineages, i.e. A.Br.001/002 (n = 4), A.Br.Ames (n = 2), A.Br.008/011 (n = 2), A.Br.005/006 (n = 2) and A.Br.Aust94 (n = 1). The match with the A.Br.Ames lineage is of particular interest as the occurrence of such lineage in Europe is demonstrated for the first time, filling an historical gap within the phylogeography of the lineage. Comparative genome analyses of these strains with 41 isolates from other parts of the world revealed that the two Danish A.Br.008/011 strains were related to the heroin-associated strains responsible for outbreaks of injection anthrax in drug users in Europe. Eight novel diagnostic SNPs that specifically discriminate the different sub-groups of Danish strains were identified and developed into PCR-based genotyping assays.

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Citations

Feb 16, 2020·BMC Infectious Diseases·Enmin ZhangJianchun Wei
Feb 21, 2021·Molecular Phylogenetics and Evolution·Sergey V PisarenkoAlexander N Kulichenko
Oct 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Farzana Islam RumeMatthias Hanczaruk
May 8, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Eugene EremenkoAlexander Kulichenko
Jul 6, 2021·Veterinary World·Bylaiah SushmaGowda Srikantha
Jun 27, 2020·European Journal of Microbiology & Immunology·Ernst-Jürgen FinkeHagen Frickmann

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Datasets Mentioned

BETA
CP001970.1
AE017334.2
AE017336.2
AE017335.3

Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

Primer
BioNumerics
MEGA6

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