Whole-Genome Sequencing of a Single Viral Species from a Highly Heterogeneous Sample

Angewandte Chemie
Hee-Sun HanDavid A Weitz

Abstract

Metagenomic studies suggest that only a small fraction of the viruses that exist in nature have been identified and studied. Characterization of unknown viral genomes is hindered by the many genomes populating any virus sample. A new method is reported that integrates drop-based microfluidics and computational analysis to enable the purification of any single viral species from a complex mixed virus sample and the retrieval of complete genome sequences. By using this platform, the genome sequence of a 5243 bp dsDNA virus that was spiked into wastewater was retrieved with greater than 96% sequence coverage and more than 99.8% sequence identity. This method holds great potential for virus discovery since it allows enrichment and sequencing of previously undescribed viruses as well as known viruses.

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Citations

Jun 14, 2016·Chemical Society Reviews·Sławomir LachBartosz A Grzybowski
Aug 18, 2017·Lab on a Chip·Jae Jung KimPatrick S Doyle
Feb 2, 2019·Scientific Reports·Kazuhito V TabataHiroyuki Noji
May 11, 2021·Analytical Chemistry·Chen SunAdam R Abate

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