Visceral leishmaniasis is a potentially fatal disease endemic to large parts of Asia and Africa, primarily caused by the protozoan parasite Leishmania donovani. Here, we report a high-quality reference genome sequence for a strain of L. donovani from Nepal, and use this sequence to study variation in a set of 16 related clinical lines, isolated from visceral leishmaniasis patients from the same region, which also differ in their response to in vitro drug susceptibility. We show that whole-genome sequence data reveals genetic structure within these lines not shown by multilocus typing, and suggests that drug resistance has emerged multiple times in this closely related set of lines. Sequence comparisons with other Leishmania species and analysis of single-nucleotide diversity within our sample showed evidence of selection acting in a range of surface- and transport-related genes, including genes associated with drug resistance. Against a background of relative genetic homogeneity, we found extensive variation in chromosome copy number between our lines. Other forms of structural variation were significantly associated with drug resistance, notably including gene dosage and the copy number of an experimentally verified circular e...Continue Reading
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Gene expression analysis of the mechanism of natural Sb(V) resistance in Leishmania donovani isolates from Nepal
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Relapse after treatment with miltefosine for visceral leishmaniasis is associated with increased infectivity of the infecting Leishmania donovani strain
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Genomic confirmation of hybridisation and recent inbreeding in a vector-isolated Leishmania population
Drug susceptibility in Leishmania isolates following miltefosine treatment in cases of visceral leishmaniasis and post kala-azar dermal leishmaniasis
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Identification of a RAC/AKT-like gene in Leishmania parasites as a putative therapeutic target in leishmaniasis
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Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Assay for Diagnosis of Cutaneous and Visceral Leishmaniasis
Modulation of Aneuploidy inLeishmania donovani during Adaptation to DifferentIn Vitro andIn Vivo Environments and Its Impact on Gene Expression
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The selenocysteine tRNA gene in leishmania major is transcribed by both RNA polymerase II and RNA polymerase III
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Genomes of Leishmania parasites directly sequenced from patients with visceral leishmaniasis in the Indian subcontinent
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Intraspecific Genomic Divergence and Minor Structural Variations in Leishmania (Viannia) panamensis.
Experimental Evidence on the Nature of the Antigen in the Direct Agglutination Test for Visceral Leishmaniasis
Identification of divergent Leishmania (Viannia) braziliensis ecotypes derived from a geographically restricted area through whole genome analysis
Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania
Major changes in chromosomal somy, gene expression and gene dosage driven by SbIII in Leishmania braziliensis and Leishmania panamensis
In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species
Antileishmanial Aminopyrazoles: Studies into Mechanisms and Stability of Experimental Drug Resistance
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Isolation and characterisation of Leishmania donovani protein antigens from urine of visceral leishmaniasis patients
Molecular mechanisms of drug resistance in natural Leishmania populations vary with genetic background
Mutation in elongation factor G confers resistance to the antibiotic argyrin in the opportunistic pathogen Pseudomonas aeruginosa
Alice in microbes' land: adaptations and counter-adaptations of vector-borne parasitic protozoa and their hosts
Genome wide comparison of Ethiopian Leishmania donovani strains reveals differences potentially related to parasite survival
Whole genome sequencing of Trypanosoma cruzi field isolates reveals extensive genomic variability and complex aneuploidy patterns within TcII DTU
Reverse Epidemiology: An Experimental Framework to Drive Leishmania Biomarker Discovery in situ by Functional Genetic Screening Using Relevant Animal Models
J -binding protein 1 and J -binding protein 2 expression in clinical Leishmania major no response-antimonial isolates
Transcriptional responses of Leishmania (Leishmania) amazonensis in the presence of trivalent sodium stibogluconate
Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia
UDP-glycosyltransferase genes in trypanosomatid genomes have diversified independently to meet the distinct developmental needs of parasite adaptations
Identification and Functional Validation of a Biomarker for the Diagnosis of Miltefosine Relapse during Visceral Leishmaniasis
Minor temperature shifts do not affect chromosomal ploidy but cause transcriptomic changes in Leishmania braziliensis promastigotes in vitro
Application of next generation sequencing (NGS) for descriptive analysis of 30 genomes of Leishmania infantum isolates in Middle-North Brazil
The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations.
Population structure and evidence for both clonality and recombination among Brazilian strains of the subgenus Leishmania (Viannia)
Antimony-Resistant Leishmania donovani Exploits miR-466i To Deactivate Host MyD88 for Regulating IL-10/IL-12 Levels during Early Hours of Infection
Population structures of Leishmania infantum and Leishmania tropica the causative agents of kala-azar in Southwest Iran
A complete Leishmania donovani reference genome identifies novel genetic variations associated with virulence
Next Generation Sequencing and Bioinformatics Methodologies for Infectious Disease Research and Public Health: Approaches, Applications, and Considerations for Development of Laboratory Capacity.
Phenotypic adaptations of Leishmania donovani to recurrent miltefosine exposure and impact on sand fly infection
Casein kinase 1.2 over expression restores stress resistance to Leishmania donovani HSP23 null mutants
Evaluation of whole genome amplification and bioinformatic methods for the characterization of Leishmania genomes at a single cell level
Transcriptional Profiling in Experimental Visceral Leishmaniasis Reveals a Broad Splenic Inflammatory Environment that Conditions Macrophages toward a Disease-Promoting Phenotype
Leishmania Genome Dynamics during Environmental Adaptation Reveal Strain-Specific Differences in Gene Copy Number Variation, Karyotype Instability, and Telomeric Amplification
Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania
Expanded genome-wide comparisons give novel insights into population structure and genetic heterogeneity of Leishmania tropica complex
Structures and stabilization of kinetoplastid-specific split rRNAs revealed by comparing leishmanial and human ribosomes
Integrative genomic, proteomic and phenotypic studies of Leishmania donovani strains revealed genetic features associated with virulence and antimony-resistance.
Trypanosoma Cruzi Genome: Organization, Multi-Gene Families, Transcription, and Biological Implications.
Analysis of the IGS rRNA Region and Applicability for Leishmania (V .) braziliensis Characterization
Nuclear and mitochondrial genome sequencing of North-African Leishmania infantum isolates from cured and relapsed visceral leishmaniasis patients reveals variations correlating with geography and phenotype
Mutations in an Aquaglyceroporin as a Proven Marker of Antimony Clinical Resistance in the Parasite Leishmania donovani.
Genomic Diversification, Structural Plasticity, and Hybridization in Leishmania (Viannia) braziliensis.
Genome Sequencing of Leishmania infantum Causing Cutaneous Leishmaniosis from a Turkish Isolate with Next-Generation Sequencing Technology.
Genomic and Transcriptomic Analysis for Identification of Genes and Interlinked Pathways Mediating Artemisinin Resistance in Leishmania donovani.
Leishmania Sexual Reproductive Strategies as Resolved through Computational Methods Designed for Aneuploid Genomes.
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A review of the systematics, species identification and diagnostics of the Trypanosomatidae using the maxicircle kinetoplast DNA: from past to present
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