Whole-genome sequencing reveals distinct mutational patterns in closely related laboratory and naturally propagated Francisella tularensis strains.

PloS One
Andreas SjödinPär Larsson

Abstract

The F. tularensis type A strain FSC198 from Slovakia and a second strain FSC043, which has attenuated virulence, are both considered to be derivatives of the North American F. tularensis type A strain SCHU S4. These strains have been propagated under different conditions: the FSC198 has undergone natural propagation in the environment, while the strain FSC043 has been cultivated on artificial media in laboratories. Here, we have compared the genome sequences of FSC198, FSC043, and SCHU S4 to explore the possibility that the contrasting propagation conditions may have resulted in different mutational patterns. We found four insertion/deletion events (INDELs) in the strain FSC043, as compared to the SCHU S4, while no single nucleotide polymorphisms (SNPs) or variable number of tandem repeats (VNTRs) were identified. This result contrasts with previously reported findings for the strain FSC198, where eight SNPs and three VNTR differences, but no INDELs exist as compared to the SCHU S4 strain. The mutations detected in the laboratory and naturally propagated type A strains, respectively, demonstrate distinct patterns supporting that analysis of mutational spectra might be a useful tool to reveal differences in past growth condition...Continue Reading

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Citations

Mar 26, 2013·Journal of Biotechnology·Minenosuke MatsutaniKazunobu Matsushita
Nov 6, 2013·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Andreas SjödinMats Forsman
Jul 19, 2011·Veterinary Microbiology·Chad R LaingVictor P J Gannon
Nov 14, 2015·Bioscience, Biotechnology, and Biochemistry·Kazunobu MatsushitaMamoru Yamada
Jun 18, 2014·Infection and Immunity·Marie LindgrenAnders Sjöstedt

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Datasets Mentioned

BETA
AJ749949.2
AAYE00000000.1
SRA009329.1

Methods Mentioned

BETA
PCR

Software Mentioned

MOSAIK
MUMmer
nucmer
Gigabayes
Exonorate
VAAL
Velvet

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