May 27, 2017

Whole genome sequencing reveals within-host genetic changes in paired meningococcal carriage isolates from Ethiopia

BMC Genomics
Guro K BårnesDominique A Caugant

Abstract

Meningococcal colonization is a prerequisite for transmission and disease, but the bacterium only very infrequently causes disease while asymptomatic carriage is common. Carriage is highly dynamic, showing a great variety across time and space within and across populations, but also within individuals. The understanding of genetic changes in the meningococcus during carriage, when the bacteria resides in its natural niche, is important for understanding not only the carriage state, but the dynamics of the entire meningococcal population. Paired meningococcal isolates, obtained from 50 asymptomatic carriers about 2 months apart were analyzed with whole genome sequencing (WGS). Phylogenetic analysis revealed that most paired isolates from the same individual were closely related, and the average and median number of allelic differences between paired isolates defined as the same strain was 35. About twice as many differences were seen between isolates from different individuals within the same sequence type (ST). In 8%, different strains were detected at different time points. A difference in ST was observed in 6%, including an individual who was found to carry three different STs over the course of 9 weeks. One individual carrie...Continue Reading

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Mentioned in this Paper

Genome-Wide Association Study
Study
Neisseria meningitidis
RELA wt Allele
Hemorrhoids
Genome
Asymptomatic Diseases
Genes
Recombination, Genetic
Phylogenetic Analysis

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