Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

Journal of Visualized Experiments : JoVE
Scott C Montgomery, Brandon C Cox

Abstract

The organ of Corti, housed in the cochlea of the inner ear, contains mechanosensory hair cells and surrounding supporting cells which are organized in a spiral shape and have a tonotopic gradient for sound detection. The mouse cochlea is approximately 6 mm long and often divided into three turns (apex, middle, and base) for analysis. To investigate cell loss, cell division, or mosaic gene expression, the whole mount or surface preparation of the cochlea is useful. This dissection method allows visualization of all cells within the organ of Corti when combined with immunostaining and confocal microscopy to image cells at different planes in the z-axis. Multiple optical cross-sections can also be obtained from these z-stack images. In addition, the whole mount dissection method can be used for scanning electron microscopy, although a different fixation method is needed. Here, we present a method to isolate the organ of Corti as three intact cochlear turns (apex, middle, and base). This method can be used for mice ranging from one week of age through adulthood and differs from the technique used for neonatal samples where calcification of the cochlea is incomplete. A slightly modified version can be used for dissection of the rat ...Continue Reading

Citations

Apr 11, 2018·Scientific Reports·Stephen T PaquettePatricia M White
Sep 19, 2019·International Journal of Environmental Research and Public Health·Sang Hyun KwakJinsei Jung
Nov 28, 2020·Scientific Reports·Z Jason Qian, Anthony J Ricci
Nov 21, 2019·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·James G NaplesDaqing Li
May 1, 2021·Life·Kathrin MalfeldVerena Scheper
Aug 3, 2021·Frontiers in Neurology·Jean-Paul BryantJared S Rosenblum
Nov 23, 2021·Frontiers in Cell and Developmental Biology·Ido BadashJohn S Oghalai

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