Whole-mount immunohistochemistry of the brain

Current Protocols in Neuroscience
Se-Hoon KimRichard Hawkes

Abstract

The gross anatomical distribution of an antigen is typically mapped using a combination of serial sectioning, immunocytochemistry, and three-dimensional reconstruction. This is a tedious and time-consuming procedure, which introduces an array of potential alignment and differential shrinkage errors and requires considerable experience and specialized equipment. In particular, it is unsuited for routine screening applications. To circumvent these problems, this unit presents a routine whole-mount immunocytochemistry protocol that can be used to map many antigenic distributions in the developing and adult brain. The technique can also be easily adapted to detect anterograde and retrograde transport tracers.

References

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Oct 22, 2003·Progress in Neurobiology·Justyna R Sarna, Richard Hawkes
Jan 22, 2005·Progress in Brain Research·Roy V SillitoeRichard Hawkes

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Citations

Aug 18, 2010·Reproductive Biology and Endocrinology : RB&E·Rajasekhar SingavarapuSandra Orsulic
Apr 18, 2012·Journal of Visualized Experiments : JoVE·Joshua J WhiteRoy V Sillitoe
Dec 5, 2009·Current Protocols in Stem Cell Biology·Jan PruszakGuido Nikkhah
Jul 6, 2019·ACS Chemical Neuroscience·Damien DepannemaeckerJean Rinkel

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