Whole organ sheep kidney tissue engineering and in vivo transplantation: Effects of perfusion-based decellularization on vascular integrity

Materials Science & Engineering. C, Materials for Biological Applications
Abdol-Mohammad KajbafzadehReza Seyed Hossein Beigi

Abstract

During the past decade, increased efforts have been made to develop alternative management options instead of dialysis and homograft renal transplantation for end-stage renal disease. State-of-the-art methods employ tissue engineering to produce natural acellular scaffolds that could resolve the concern of allograft rejection and obviate the need for immunosuppressive therapy. Complete decellularization of kidney with intact extracellular matrix is crucial for in vivo compatibility and success of transplantation. Herein, we evaluate the efficacy of two different whole organ decellularization protocols, vasculature integrity, and in vivo transplantation of sheep kidneys. Eight sheep kidneys were decellularized by perfusion-based method utilizing two different protocols (Protocol 1: 1% Triton X-100 and 0.5% SDS vs. Protocol 2: 1% SDS). The samples were evaluated by histopathology in terms of decellularization and extracellular matrix preservation. Computerized tomography angiography was performed to evaluate vasculature. Subsequently, both methods were transplanted in four sheep and monitored for vascular integrity and extravasations in short-term. Scaffolds obtained from both protocols were entirely decellularized. However; the ...Continue Reading

Citations

Sep 10, 2020·Frontiers in Bioengineering and Biotechnology·Iris RibitschLouis C Penning
Oct 22, 2020·Medical Engineering & Physics·Dario CarbonaroDiana Massai
May 21, 2021·Tissue Engineering and Regenerative Medicine·Panagiotis MallisMichalis Katsimpoulas

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