Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT.

ELife
Spencer R KatzKeith C Cheng

Abstract

We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables.

References

Jan 1, 1989·Developmental Biology·G StreisingerD J Grunwald
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Aug 1, 1997·Radiology·W S EnochsR Weissleder
May 18, 2001·BioTechniques·J R TrueS B Carroll
Sep 21, 2002·Microscopy Research and Technique·James A Lister
Jul 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Masashi HirataShigeru Kondo
Jul 15, 2004·Pigment Cell Research·Robert N Kelsh
Sep 24, 2004·Physiological Reviews·Andrzej SlominskiJacobo Wortsman
Aug 20, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Masashi HirataShigeru Kondo
May 18, 2006·Pigment Cell Research·Darren W LoganIan J Jackson
Jul 13, 2006·Heredity·D M Parichy
Aug 5, 2006·Genetics·Jessica L MooreKeith C Cheng
Apr 19, 2007·Nature Reviews. Genetics·Graham J Lieschke, Peter D Currie
Mar 29, 2008·Cell Stem Cell·Richard Mark WhiteLeonard I Zon
May 27, 2008·Nature Biotechnology·Yannick DoyonSharon L Amacher
May 13, 2009·Optics Express·Beat MünchMarco Stampanoni
Sep 18, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Gang RenZhen Cheng
May 20, 2011·Developmental Neurobiology·Gaia GestriStephan C F Neuhauss
Sep 9, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brian D Metscher, Gerd B Müller
Oct 4, 2011·Current Opinion in Genetics & Development·Keith C ChengPatrick La Riviere
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 4, 2013·Development·Hans Georg FrohnhöferChristiane Nüsslein-Volhard
Sep 2, 2014·Journal of Synchrotron Radiation·Dogˇa GürsoyChris Jacobsen
Oct 25, 2014·Journal of Synchrotron Radiation·E X MiquelesJ S V Bermúdez
Jan 21, 2015·Current Biology : CB·Ajeet Pratap Singh, Christiane Nüsslein-Volhard
Sep 17, 2015·Mechanisms of Development·Moritz KneippDaniel Razansky
Mar 24, 2016·Developmental Cell·Kazuyuki HoshijimaDavid Jonah Grunwald
Jul 7, 2016·Scientific Reports·Paride Antinucci, Robert Hindges
Aug 2, 2017·Developmental Biology·Gianluca D'AgatiRichard M White
Aug 9, 2018·Development, Growth & Differentiation·Yuu UsuiMasakatsu Watanabe
Jul 28, 2020·ELife·Jennifer P OwenChristian A Yates

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