We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables.
Pigment cell distributions in different tissues of the zebrafish, with special reference to the striped pigment pattern
The other pigment cell: specification and development of the pigmented epithelium of the vertebrate eye
SLC24A5 encodes a trans-Golgi network protein with potassium-dependent sodium-calcium exchange activity that regulates human epidermal melanogenesis
Quantitative imaging of element composition and mass fraction using dual-energy CT: three-material decomposition.
Differential contribution of direct-developing and stem cell-derived melanocytes to the zebrafish larval pigment pattern.
MicroCT for molecular imaging: quantitative visualization of complete three-dimensional distributions of gene products in embryonic limbs
Iridophores and their interactions with other chromatophores are required for stripe formation in zebrafish
Volumetric tracking of migratory melanophores during zebrafish development by optoacoustic microscopy
Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography.
SATB2 induction of a neural crest mesenchyme-like program drives melanoma invasion and drug resistance.
CZI Human Cell Atlas Seed Network
The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.