Whole Sequences and Characteristics of mcr-1-Harboring Plasmids of Escherichia coli Strains Isolated from Livestock in South Korea

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Ji-Young LeeKwan Soo Ko

Abstract

Of 11 mcr-1-harboring plasmids previously identified from livestock in Korea, we performed whole plasmid sequencing on 3 plasmids and determined the genetic structure surrounding mcr-1 for all 11 plasmids. Transconjugation frequencies were measured for all mcr-1-harboring plasmids and competitive growth experiments were performed to investigate the fitness cost of each plasmid. Although they belong to different clones, the mcr-1-harboring plasmids, pEC006 and pEC019, were highly similar to the first identified mcr-1-carrying Incl2-type plasmid, pHNSHP45. Another IncX4-type plasmid, pEC111, had completely different structure from these plasmids, but was similar to pMCR1-IncX4. A nearly identical 11.3 kb mcr-1 region (nikB-ISApl1-mcr-1-pap2-topB) was shared by all mcr-1-harboring plasmids except pEC111. The transfer rate of mcr-1-harboring plasmids was highly variable (10-11 to 10-3) and was not related to plasmid structure. Competitive growth experiments revealed that the fitness of all three transconjugants with mcr-1-harboring plasmids increased compared with that of the recipient strain, Escherichia coli J53. The mcr-1-harboring plasmids may have been repeatedly introduced into bacterial isolates since the initial introductio...Continue Reading

References

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Citations

Sep 23, 2020·Journal of Microbiology and Biotechnology·Seokhwan KimSangryeol Ryu

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Methods Mentioned

BETA
PCR

Software Mentioned

BLAST

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