Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Host's Immune Response to Coccidiosis

Frontiers in Physiology
Lijin GuoQinghua Nie

Abstract

Coccidiosis is a major hazard to the chicken industry, but the host's immune response to coccidiosis remains unclear. Here, we performed Eimeria coccidia challenge in 28-day-old ROSS 308 broilers and selected the bursa from the three most severely affected individuals and three healthy individuals for RNA sequencing. We obtained 347 DEGs from RNA-seq and found that 7 upregulated DEGs were enriched in Cytokine-cytokine receptor interaction pathway. As the DEGs with the highest expression abundance in these 7 genes, TNFRSF6B was speculated to participate in the process of host's immune response to coccidiosis. It is showed that TNFRSF6B can polarize macrophages to M1 subtype and promote inflammatory cytokines expression. In addition, the expression of TNFRSF6B suppressed HD11 cells apoptosis by downregulating Fas signal pathway. Besides, TNFRSF6B-mediated macrophages immunity activation can be reversed by apoptosis. Overall, our study indicates that TNFRSF6B upregulated in BAE, is capable of aggravating the inflammatory response by inhibiting macrophages apoptosis via downregulating Fas signal pathway, which may participate in host's immune response to coccidiosis.

References

Mar 16, 2000·Developmental and Comparative Immunology·C H YunE P Lillehoj
Dec 6, 2003·Journal of Leukocyte Biology·Yung-Chi ChangShie-Liang Hsieh
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lisa RothwellPete Kaiser
Oct 19, 2004·Seminars in Cancer Biology·Mary PhilipHans Schreiber
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chia-Ron YangWan-Wan Lin
Aug 17, 2005·Current Drug Targets. Inflammation and Allergy·Nagatoshi Fujiwara, Kazuo Kobayashi
Oct 19, 2005·Advances in Parasitology·Martin W ShirleyFiona M Tomley
Feb 3, 2006·Expert Review of Vaccines·Rami A Dalloul, Hyun S Lillehoj
Jan 15, 2008·Clinical and Experimental Immunology·C-S LeeP-N Hsu
Sep 2, 2008·Nature Genetics·Subra KugathasanHakon Hakonarson
Sep 15, 2010·Inflammatory Bowel Diseases·Paul HendersonRichard K Russell
Mar 23, 2011·Osteoarthritis and Cartilage·S HayashiR Kuroda
Apr 16, 2013·Developmental and Comparative Immunology·Wongi MinHyun S Lillehoj
Nov 19, 2013·Trends in Parasitology·Damer P Blake, Fiona M Tomley
Feb 28, 2014·Veterinary Research·Emilio Del CachoCaridad Sánchez-Acedo
Jul 10, 2014·Current Topics in Microbiology and Immunology·Abhisake Kole, Kevin J Maloy
Dec 18, 2014·International Journal for Parasitology. Drugs and Drug Resistance·H David Chapman, Thomas K Jeffers
Mar 19, 2015·BioMed Research International·Rosa Estela Quiroz-Castañeda, Edgar Dantán-González
Jun 24, 2015·Biochemical and Biophysical Research Communications·Yi ZhangJian Zhou
Jun 21, 2017·Journal of Biomedical Science·Shie-Liang Hsieh, Wan-Wan Lin
Oct 24, 2017·The Journal of Pathology·Hsiao-Wen TsaiShie-Liang Hsieh
Nov 23, 2018·Genetics, Selection, Evolution : GSE·Kay BoultonDavid A Hume
Sep 15, 2019·Veterinary Immunology and Immunopathology·Maria ArendtLaura J Knoll

❮ Previous
Next ❯

Citations

Feb 28, 2021·Journal of Applied Genetics·Lintong LuoQianning Wang
Aug 18, 2021·Animal Reproduction Science·Mawahib K KhogaliTuoyu Geng

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA561064

Methods Mentioned

BETA
PCR
transfection
immunoprecipitation
Fluorescence
RNA-seq
ELISA

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Ugeskrift for laeger
P Faurschou
Annual Review of Microbiology
N D LEVINE
Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Wei-Cheng TsengDer-Cherng Tarng
The Canadian Veterinary Journal. La Revue Vétérinaire Canadienne
L J Weber
Canadian Journal of Comparative Medicine and Veterinary Science
A B Wickware
© 2021 Meta ULC. All rights reserved