Whole-Transcriptome Analysis of Verocytotoxigenic Escherichia coli O157:H7 (Sakai) Suggests Plant-Species-Specific Metabolic Responses on Exposure to Spinach and Lettuce Extracts

Frontiers in Microbiology
Louise CrozierNicola Holden

Abstract

Verocytotoxigenic Escherichia coli (VTEC) can contaminate crop plants, potentially using them as secondary hosts, which can lead to food-borne infection. Currently, little is known about the influence of the specific plant species on the success of bacterial colonization. As such, we compared the ability of the VTEC strain, E. coli O157:H7 'Sakai,' to colonize the roots and leaves of four leafy vegetables: spinach (Spinacia oleracea), lettuce (Lactuca sativa), vining green pea (Pisum sativum), and prickly lettuce (Lactuca serriola), a wild relative of domesticated lettuce. Also, to determine the drivers of the initial response on interaction with plant tissue, the whole transcriptome of E. coli O157:H7 Sakai was analyzed following exposure to plant extracts of varying complexity (spinach leaf lysates or root exudates, and leaf cell wall polysaccharides from spinach or lettuce). Plant extracts were used to reduce heterogeneity inherent in plant-microbe interactions and remove the effect of plant immunity. This dual approach provided information on the initial adaptive response of E. coli O157:H7 Sakai to the plant environment together with the influence of the living plant during bacterial establishment and colonization. Results...Continue Reading

Citations

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1982·Journal of Bacteriology·D A RatkowskyA Ball
Apr 1, 1994·Molecular Microbiology·Michael A QuailJ R Guest
Apr 1, 1996·The Australian and New Zealand Journal of Surgery·F Chen, M Stuart
Dec 24, 1997·Molecular Microbiology·R SchneiderG Muskhelishvili
Mar 4, 1998·Molecular Microbiology·K YamanakaMasayori Inouye
Sep 6, 2002·Microbiology·Tatiana UmanskiDevorah Friedberg
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Oct 7, 2003·Research in Microbiology·Alessandra PolissiGianni Dehò
Feb 10, 2004·Applied and Environmental Microbiology·Maria T BrandlRobert E Mandrell
Jun 9, 2004·Journal of Medical Microbiology·Nicola Holden, David L Gally
Apr 6, 2006·Journal of Bacteriology·Balakrishnan VenkateshShinji Tsuyumu
May 18, 2006·Annual Review of Phytopathology·Maria T Brandl
Aug 15, 2006·Molecular Plant-microbe Interactions : MPMI·Mónica Rosenblueth, Esperanza Martínez-Romero
Sep 4, 2008·Genes & Development·Christina PesaventoRegine Hengge
Jan 31, 2009·International Journal of Food Microbiology·J D Greig, A Ravel
Apr 7, 2009·Applied and Environmental Microbiology·Teresa M BergholzThomas S Whittam
Jan 12, 2010·Applied and Environmental Microbiology·Jennifer L KyleMaria T Brandl
Feb 16, 2010·Applied and Environmental Microbiology·Fiona P BrennanVincent O'Flaherty
May 13, 2010·Molecular Systems Biology·Szymon JozefczukLothar Willmitzer
Jun 5, 2010·Journal of Applied Microbiology·R Van Houdt, C W Michiels
Jan 12, 2011·Methods in Molecular Biology·Zoë A Popper
Apr 12, 2011·Applied and Environmental Microbiology·Michelle Q CarterRobert E Mandrell
May 17, 2011·Food Microbiology·Yulia KroupitskiShlomo Sela
Oct 11, 2011·International Journal of Medical Microbiology : IJMM·Christian-Daniel Köhler, Ulrich Dobrindt
Jan 17, 2012·Applied and Environmental Microbiology·Ryan C FinkFrancisco Diez-Gonzalez
Feb 10, 2012·Foodborne Pathogens and Disease·Dumitru MacarisinVijay K Sharma

Related Concepts

Microorganism
Metabolic Process, Cellular
Spinach preparation
Biological Adaptation to Stress
Lettuce
Green Leafy Vegetable
Lactuca sativa
Lactuca serriola
Spinacia oleracea antigen
Crops, Agricultural

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