Whole-Transcriptome Analysis Unveils the Synchronized Activities of Genes for Fructans in Developing Tubers of the Jerusalem Artichoke

Frontiers in Plant Science
Marco BizzarriFrancesco Paolocci

Abstract

Helianthus tuberosus L., known as the Jerusalem artichoke, is a hexaploid plant species, adapted to low-nutrient soils, that accumulates high levels of inulin in its tubers. Inulin is a fructose-based polysaccharide used either as dietary fiber or for the production of bioethanol. Key enzymes involved in inulin biosynthesis are well known. However, the gene networks underpinning tuber development and inulin accumulation in H. tuberous remain elusive. To fill this gap, we selected 6,365 expressed sequence tags (ESTs) from an H. tuberosus library to set up a microarray platform and record their expression across three tuber developmental stages, when rhizomes start enlarging (T0), at maximum tuber elongation rate (T3), and at tuber physiological maturity (Tm), in "VR" and "K8-HS142"clones. The former was selected as an early tuberizing and the latter as a late-tuberizing clone. We quantified inulin and starch levels, and qRT-PCR confirmed the expression of critical genes accounting for inulin biosynthesis. The microarray analysis revealed that the differences in morphological and physiological traits between tubers of the two clones are genetically determined since T0 and that is relatively low the number of differentially expres...Continue Reading

References

Sep 1, 1991·The Plant Cell·J DeneckeJ Botterman
Jul 1, 1991·The Plant Cell·D Van der StraetenM Van Montagu
Mar 19, 1970·Biochimica Et Biophysica Acta·M D BickB L Strehler
Dec 1, 1994·Plant Physiology·J M BoyceD E Evans
Jan 1, 1995·The Plant Journal : for Cell and Molecular Biology·R ZrennerU Sonnewald
Nov 1, 1996·The Plant Journal : for Cell and Molecular Biology·C LinA R Cashmore
Oct 1, 1998·The Plant Journal : for Cell and Molecular Biology·I M van der MeerA J van Tunen
Oct 24, 1998·Cell Motility and the Cytoskeleton·R C MooreR J Cyr
Jun 11, 1999·Plant Physiology·I Vijn, S Smeekens
Jul 8, 1999·The Journal of Nutrition·L Prosky, H Hoebregs
Aug 11, 2001·Critical Reviews in Food Science and Nutrition·G FlammM Roberfroid
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Feb 1, 2002·Developmental Biology·José Manuel Pérez-PérezJosé Luis Micol
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·Barbara Fleck, Nicholas P Harberd
Aug 13, 2003·Plant Physiology·Aaron M RashotteJoseph J Kieber
May 22, 2004·European Journal of Biochemistry·Isaura Simões, Carlos Faro
Dec 8, 2004·The Plant Cell·Jennifer MoonMark Estelle
Aug 24, 2005·Nature Structural & Molecular Biology·Stephane R Gross, Terri Goss Kinzy
Feb 2, 2006·BMC Molecular Biology·Andreas SchroederThomas Ragg
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Jul 1, 1957·Plant Physiology·G Miller, H J Evans
Aug 19, 2006·Applied Biochemistry and Biotechnology·Ma José NegroMercedes Ballesteros
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa

❮ Previous
Next ❯

Citations

Jun 3, 2021·International Journal of Molecular Sciences·Silin WuHongbo Zhao
Nov 9, 2021·Frontiers in Plant Science·Alma CostarelliStefania Pasqualini

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE132955
AJ009757.1

Methods Mentioned

BETA
PCR
Chip
protein folding
RNA-seq

Software Mentioned

LIMMA
OligoArray
CombiMatrix
R
OligoExpress
Cell [UNK]
BlastN
Statistical Package for Social Sciences ( SPSS
Microarray Imager
KeGG

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.