Whole-transcriptome changes in gene expression accompany aging of sensory neurons in Aplysia californica

BMC Genomics
Justin B GreerLynne A Fieber

Abstract

Large-scale molecular changes occur during aging and have many downstream consequences on whole-organism function, such as motor function, learning, and memory. The marine mollusk Aplysia californica can be used to study transcriptional changes that occur with age in identified neurons of the brain, because its simplified nervous system allows for more direct correlations between molecular changes, physiological changes, and their phenotypic outcomes. Behavioral deficits in the tail-withdrawal reflex of aged animals have been correlated with reduced excitation in sensory neurons that control the reflex. RNASeq was used to investigate whole-transcriptome changes in tail-withdrawal sensory neurons of sexually mature and aged Aplysia to correlate transcriptional changes with reduced behavioral and physiological responses. Paired-end sequencing resulted in 210 million reads used for differential expression analysis. Aging significantly altered expression of 1202 transcripts in sensory neurons underlying the tail-withdrawal reflex, with an approximately equal number of these genes up- and down regulated with age. Despite overall bidirectionality of expression changes, > 80% of ion channel genes that were differentially expressed had...Continue Reading

References

Mar 1, 1982·Journal of Neurobiology·B PeretzR Wilson
Feb 10, 1999·Experimental Gerontology·S M McCannV Rettorri
Jul 11, 2000·Nature Genetics·C K LeeT A Prolla
Jan 25, 2002·Annals of the New York Academy of Sciences·E R Stadtman
Oct 24, 2002·Neurobiology of Aging·Lucio AnnunziatoMaurizio Taglialatela
Jun 11, 2004·Nature·Tao LuBruce A Yankner
Feb 12, 2005·Archives of Physical Medicine and Rehabilitation·Sun G ChungLi-Qun Zhang
Aug 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Trent NguyenStephen M Massa
Oct 26, 2005·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Sheng Zhao, Russell D Fernald
Jan 10, 2006·Annals of the New York Academy of Sciences·S M McCannV Rettori
Sep 15, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jan H SchefeHeiko Funke-Kaiser
Dec 28, 2006·Cell·Leonid L MorozEric R Kandel
Apr 7, 2007·Annals of the New York Academy of Sciences·Maureen WallaceVictoria Luine
Jun 5, 2007·Alzheimer Disease and Associated Disorders·Mark H Tuszynski
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Oct 3, 2008·PloS One·Patrick M LoerchBruce A Yankner
Feb 3, 2009·The Journal of Biological Chemistry·Truyen NguyenCecil B Pickett
Aug 22, 2009·Redox Report : Communications in Free Radical Research·Kenneth J RodgersUlf T Brunk
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jennifer BradfordShihua Li
Mar 26, 2010·Nature·Nicholas A BishopBruce A Yankner
May 11, 2010·Brain Research·Lynne A FieberMichael C Schmale
Jun 4, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dani DumitriuJohn H Morrison
Jun 22, 2010·Bioinformatics·Daniel BlankenbergUNKNOWN Galaxy Team
Mar 29, 2011·Learning & Memory·Rong-Yu LiuJohn H Byrne
Nov 19, 2011·Nucleic Acids Research·Sarah HunterSiew-Yit Yong
Aug 17, 2012·Journal of Psychiatry & Neuroscience : JPN·Timothy J RevettSatyabrata Kar
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Jun 8, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Ayesha MurshidStuart K Calderwood
Jun 12, 2013·Cell·Carlos López-OtínGuido Kroemer
Dec 18, 2013·BMC Genomics·Beena M KadakkuzhaSathyanarayanan V Puthanveettil
Jan 5, 2014·PloS One·Komolitdin AkhmedovSathyanarayanan V Puthanveettil
May 23, 2014·Frontiers in Aging Neuroscience·Andrew T Kempsell, Lynne A Fieber
Sep 12, 2014·Journal of Cell Communication and Signaling·Rehana K Leak

❮ Previous
Next ❯

Methods Mentioned

BETA
RNASeq
dissection
PCR
PCA

Software Mentioned

HTSeq
- count
Blast2GO
Galaxy
IPA
InterPro
STAR aligner
Real
Ingenuity Pathways Analysis ( IPA )
BLAST

Related Concepts

Related Feeds

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.

Aging Genetics (Keystone)

This feed focuses on aging epidemiology and genetic, epigenetic, and proteomic aspects underlying aging, as well as aging- associated biomarkers. Here the latest research in this domain.

Brain Aging

Here is the latest research on intrinsic and extrinsic factors, as well as pathways and mechanisms that underlie aging in the central nervous system.