Whole Transcriptome Screening Reveals Myelination Deficits in Dysplastic Human Temporal Neocortex

Cerebral Cortex
Catharina DonkelsCarola A Haas

Abstract

Focal cortical dysplasias (FCDs) are local malformations of the human neocortex with strong epileptogenic potential. To investigate the underlying pathomechanisms, we performed a whole human transcriptome screening to compare the gene expression pattern of dysplastic versus nondysplastic temporal neocortex. Tissue obtained from FCD IIIa cases (mean age 20.5 years) who had undergone surgical treatment, due to intractable epilepsy, was compared with nondysplastic specimens (mean age 19.9 years) by means of Affymetrix arrays covering 28 869 genes. We found 211 differentially expressed genes (DEX) among which mainly genes important for oligodendrocyte differentiation and myelination were downregulated in FCD IIIa. These findings were confirmed as functionally important by Database for Annotation, Visualization, and Integrated Discovery (DAVID) analysis. The reduced expression of myelin-associated transcripts was confirmed for FCD Ia, IIa, and IIIa by real-time RT-qPCR. In addition, we found that the density of myelin basic protein mRNA-expressing oligodendrocytes and of 2',3'-cyclic nucleotide 3'-phosphodiesterase-positive myelin fibers was significantly reduced in dysplastic cortex. Moreover, high-resolution confocal imaging and 3...Continue Reading

References

Nov 1, 1991·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·A PalminiR Ethier
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L A Sternberger, N H Sternberger
Nov 1, 1996·Journal of Clinical Neurophysiology : Official Publication of the American Electroencephalographic Society·A J Barkovich, R I Kuzniecky
May 31, 2001·Epilepsia·D C TaylorJ Phillips
Jun 13, 2002·Epilepsia·Jan KassubekAndreas Schulze-Bonhage
May 24, 2003·Cerebral Cortex·Yue Hua, Peter B Crino
Dec 4, 2003·Epilepsy Research·Seung-Ki KimByung-Kyu Cho
Mar 24, 2004·Neurology·A PalminiH V Vinters
Jan 20, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Damian BrockschniederElior Peles
Feb 17, 2006·Neurobiology of Disease·Dominique ArionJavier DeFelipe
May 23, 2006·Brain : a Journal of Neurology·Susanne FauserAndreas Schulze-Bonhage
Aug 17, 2006·Annals of Neurology·M Cecilia LjungbergGabriella D'Arcangelo
Oct 27, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jason C DugasBen A Barres
Jan 4, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John D CahoyBen A Barres
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Apr 23, 2010·Acta Neuropathologica·George HadjivassiliouMaria Thom
Mar 24, 2011·AJR. American Journal of Roentgenology·Paul A M HofmanGraeme Jackson
Jul 19, 2012·Journal of Neuropathology and Experimental Neurology·Takafumi SakakibaraMasayuki Itoh
Sep 14, 2012·Epilepsia·Jason S Hauptman, Gary W Mathern
Sep 8, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matthias KoenningBen Emery
Oct 12, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Thomas L BeaumontJeffrey A Loeb
Jun 11, 2014·Acta Neuropathologica·I Lorena Arancibia-Carcamo, David Attwell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.