Why are proteins charged? Networks of charge-charge interactions in proteins measured by charge ladders and capillary electrophoresis

Angewandte Chemie
Irina GitlinGeorge M Whitesides

Abstract

Almost all proteins contain charged amino acids. While the function in catalysis or binding of individual charges in the active site can often be identified, it is less clear how to assign function to charges beyond this region. Are they necessary for solubility? For reasons other than solubility? Can manipulating these charges change the properties of proteins? A combination of capillary electrophoresis (CE) and protein charge ladders makes it possible to study the roles of charged residues on the surface of proteins outside the active site. This method involves chemical modification of those residues to generate a large number of derivatives of the protein that differ in charge. CE separates those derivatives into groups with the same number of modified charged groups. By studying the influence of charge on the properties of proteins using charge ladders, it is possible to estimate the net charge and hydrodynamic radius and to infer the role of charged residues in ligand binding and protein folding.

References

Mar 1, 1991·Rapid Communications in Mass Spectrometry : RCM·J A LooR D Smith
Jan 1, 1991·Annual Review of Biophysics and Biophysical Chemistry·H S Chan, K A Dill
Nov 4, 1991·FEBS Letters·R Lavecchia, M Zugaro
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D StigterK A Dill
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y GotoA L Fink
Apr 10, 1990·Biochemistry·Y GotoA L Fink
Jan 1, 1990·Annual Review of Biophysics and Biophysical Chemistry·K A Sharp, B Honig
Aug 7, 1990·Biochemistry·K A Dill
Jan 1, 1989·Proteins·S J Wearne, T E Creighton
Jan 1, 1988·Advances in Protein Chemistry·P L Privalov, S J Gill
Aug 5, 1987·Journal of Molecular Biology·S MillerC Chothia
Nov 1, 1986·American Journal of Diseases of Children·E S Hill
Nov 1, 1985·Quarterly Reviews of Biophysics·K D Collins, M W Washabaugh
Feb 15, 1973·European Journal of Biochemistry·L Polgár
Jan 1, 1970·Annual Review of Biochemistry·A N Glazer
May 23, 1972·Biochemistry·C Tanford, R Roxby
Mar 4, 1982·Biochimica Et Biophysica Acta·M Hollecker, T E Creighton
Jun 5, 1982·Journal of Molecular Biology·J Warwicker, H C Watson
Mar 1, 1980·Journal of Biochemistry·S KuramitsuK Nakashima
Dec 15, 1995·Journal of Molecular Biology·Y J TanA R Fersht
Apr 1, 1995·Protein Science : a Publication of the Protein Society·K A DillH S Chan
Jun 1, 1995·Biophysical Journal·S A Allison, V T Tran
Jul 7, 1995·Science·A WarshelP A Kollman
May 26, 1995·Science·B Honig, A Nicholls
Apr 29, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A R Fersht
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·J GaoG M Whitesides
Feb 1, 1994·Protein Science : a Publication of the Protein Society·Z S Hendsch, B Tidor
Jun 24, 1994·Science·W W Cleland, M M Kreevoy
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S SimonM Schindler
Jul 1, 1993·Protein Science : a Publication of the Protein Society·A L FinkS A Swedberg
May 20, 1993·Journal of Molecular Biology·A S Yang, B Honig
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C N PaceK Gajiwala

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Citations

May 27, 2011·Amino Acids·Ivano EberiniElisabetta Gianazza
Nov 28, 2012·Biosensors & Bioelectronics·Jie ChouJohn T McDevitt
Jan 29, 2010·Journal of the American Society for Mass Spectrometry·Kiran Kumar MorishettiJianhua Ren
Jul 5, 2006·Quarterly Reviews of Biophysics·George M Whitesides, Vijay M Krishnamurthy
Jul 25, 2013·Quarterly Reviews of Biophysics·Mikael Lund, Bo Jönsson
Oct 26, 2012·Journal of the American Chemical Society·Bryan F ShawGeorge M Whitesides
Nov 28, 2008·Journal of the American Chemical Society·Grégory F SchneiderGeorge M Whitesides
Mar 31, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Hongkwan Cho, Yan Zhao
Aug 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Andrew LeeGeorge M Whitesides
Mar 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Martin KurnikMikael Oliveberg
Dec 23, 2008·BMC Genomics·Judith HübscherPatricia Stutzmann Meier
Jun 16, 2006·The Journal of Chemical Physics·Y S Velichko, M Olvera de la Cruz
Mar 3, 2012·The Journal of Chemical Physics·Dmitry V Matyushov
Feb 5, 2009·The Journal of Chemical Physics·William KungMonica Olvera de la Cruz
Dec 30, 2014·BMC Biotechnology·Karin BuettnerThole Zuchner
Sep 2, 2015·Scientific Reports·Javier MartínezMaría Gasset
Jul 25, 2015·ACS Chemical Neuroscience·Yunhua ShiBryan F Shaw
Feb 2, 2016·Journal of Biomolecular Structure & Dynamics·Danish IdreesMd Imtaiyaz Hassan
Apr 8, 2016·Journal of the American Society for Mass Spectrometry·Rachel R Ogorzalek Loo, Joseph A Loo
Jun 14, 2016·The Journal of Physical Chemistry. B·Kyungtae KangGeorge M Whitesides
Aug 12, 2016·The Journal of Physical Chemistry. B·Kingshuk GhoshKen A Dill
Sep 27, 2015·Journal of Molecular Biology·Cardine N NokweJohannes Buchner
Mar 20, 2016·Enzyme and Microbial Technology·Ankit P SudhirR B Subramanian
Nov 5, 2016·Protein Science : a Publication of the Protein Society·Ciara KynePeter B Crowley
Jan 5, 2016·Structure·Adam M R de GraffKen A Dill
Mar 30, 2017·Lab on a Chip·Joo Yong SimJae-Woong Jeong
Nov 3, 2015·Physical Chemistry Chemical Physics : PCCP·S HeilesEvan R Williams
Feb 17, 2018·Angewandte Chemie·Collin T ZahlerBryan F Shaw
Feb 28, 2018·Biomaterials Science·Chen LiPeng Yang

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