Why are triploid zebrafish all male?

Molecular Reproduction and Development
Thomas A Delomas, Konrad Dabrowski

Abstract

Adult triploid zebrafish Danio rerio has previously been reported to be all male. This phenomenon has only been reported in one other gonochoristic fish species, the rosy bitterling Rhodeus ocellatus, despite the fact that triploidy is induced in numerous species. To investigate the mechanism responsible, we first produced triploid zebrafish and observed gonad development. Histological sections of juvenile triploid gonads showed that primary growth oocytes were able to develop in the juvenile ovary, but no cortical alveolus or more advanced oocytes were found. All adult triploids examined were male (n = 160). Male triploids were able to induce oviposition by diploid females during natural spawning trials, but fertilization rates were low (1.0 ± 3.1%) compared with diploid male siblings (67.4 ± 16.6%). The embryos produced by triploid sires were aneuploid with a mean ploidy of 2.4 ± 0.1n, demonstrating that triploid males produce aneuploid spermatozoa. After confirming that adult triploids are all male, we produced an additional batch of triploid zebrafish and exposed them (and a group of diploid siblings) to 100 ng/L estradiol (E2) from 5 to 28 dpf. The E2 treated triploids and nontreated triploids were all male. The nontreated...Continue Reading

References

Mar 9, 2004·Aquatic Toxicology·Martina Fenske, Helmut Segner
Dec 18, 2004·Toxicologic Pathology·Igor V MizgireuvSergei Y Revskoy
Oct 22, 2008·Developmental Biology·Kellee R Siegfried, Christiane Nüsslein-Volhard
Dec 5, 2009·Methods in Molecular Biology·Tim D HewitsonGavin J Becker
Jul 28, 2010·PLoS Genetics·Adriana Rodríguez-MaríJohn H Postlethwait
Sep 29, 2011·G3 : Genes - Genomes - Genetics·Kevin M BradleyJeffrey R Smith
Apr 17, 2012·PloS One·Woei Chang LiewLaszlo Orban
Jan 26, 2013·Developmental Biology·Daniel B DranowBruce W Draper
Nov 1, 2013·Biology of Reproduction·Rajini SreenivasanLászló Orbán
Mar 1, 1989·Fish Physiology and Biochemistry·T J BenfeyE M Donaldson
Sep 1, 1982·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D Chourrout, E Quillet
Dec 3, 2013·Scientific Reports·Kanae TakatsuToshinobu Tokumoto
Sep 23, 2014·Genetics·Catherine A WilsonJohn H Postlethwait
Nov 25, 2016·Biology Letters·Thomas A Delomas, Konrad Dabrowski
Jan 14, 2017·The Journal of Experimental Biology·Laia RibasFrancesc Piferrer
Feb 28, 2018·Zebrafish·Konrad Dabrowski, Mackenzie Miller
Nov 1, 1993·Journal of Morphology·Kelly SelmanXiaoping Qi

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Citations

Apr 21, 2020·Marine Biotechnology·Liangyue PengYamei Xiao
Jul 14, 2021·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Christopher D SmallTillmann J Benfey
Sep 22, 2021·Journal of Fish Biology·Thomas A Delomas, Konrad Dabrowski

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