Wide-bore hydrodynamic chromatography in sub-second range

Journal of Separation Science
Ryoji UmeharaTetsuo Okada

Abstract

Wide-bore hydrodynamic chromatography (HDC) is a very simple method based on the passage of analytes through an open capillary. As shown in previous papers, an analyte, which diffuses over the entire cross-section of a capillary before it is detected, gives a Gaussian-shaped peak; such an analyte is called diffusive. In contrast, an analyte with poor diffusivity gives an asymmetrical peak with the apex at the half time of the peak of a diffusive analyte. Their diffusivity is determined by the diffusion coefficients, dimensions of a capillary, and a flow rate. A consideration of the diffusivity evaluated on the basis of these variables allows us to realize fast wide-bore HDC separation. Sub-second separation is demonstrated using test analytes, such as fluorescein and polystyrene latexes.

References

Jul 26, 2002·Analytical Chemistry·Emil ChmelaAlbert van den Berg
Dec 13, 2003·Analytical Chemistry·Marko T BlomAlbert van den Berg
May 26, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Makoto HaradaTetsuo Okada
Sep 15, 2005·Analytical Chemistry·Tetsuo OkadaTomoo Kido

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Citations

Feb 15, 2011·Analytical and Bioanalytical Chemistry·Petr S FedotovBoris Ya Spivakov
Mar 19, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Naoya JinnoKazuhiko Tsukagoshi
Apr 14, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ryoji UmeharaTetsuo Okada
Jun 15, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Seiji IshimotoKazuhiko Tsukagoshi
Jun 13, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuji MasuharaKazuhiko Tsukagoshi
Jun 13, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Naoya JinnoKazuhiko Tsukagoshi
Aug 17, 2016·Analytical Chemistry·M Farooq WahabDaniel W Armstrong
Nov 13, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yuji KawaiKazuhiko Tsukagoshi
Sep 15, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Akihisa Miyagawa, Tetsuo Okada
Feb 14, 2018·Analytical Chemistry·Darshan C PatelDaniel W Armstrong

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