Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy

Optics Letters
Daryl LimJerome Mertz

Abstract

We describe a method of obtaining optical sectioning with a standard wide-field fluorescence microscope. The method involves acquiring two images, one with nonuniform illumination (in our case, speckle) and another with uniform illumination (in our case, randomized speckle). An evaluation of the local contrast in the speckle-illumination image provides an optically sectioned image with low resolution. This is complemented with high-resolution information obtained from the uniform-illumination image. A fusion of both images leads to a full resolution image that is optically sectioned across all spatial frequencies. This hybrid illumination method is fast, robust, and generalizable to a variety of illumination and imaging configurations.

References

Jan 5, 2006·Optics Letters·Cathie Ventalon, Jerome Mertz
Jun 5, 2007·Optics Letters·Cathie VentalonJerome Mertz

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Citations

Dec 26, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·X MichaletS Cova
Mar 10, 2010·Journal of Biomedical Optics·Jerome Mertz, Jinhyun Kim
Oct 30, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Heejin ChoiPeter T C So
Apr 3, 2012·Journal of Biomedical Optics·Tim N FordJerome Mertz
Jun 7, 2011·Biophysical Journal·Eleonora MuroVincent Loriette
Dec 2, 2015·PloS One·Marcel A LauterbachValentina Emiliani
Jun 17, 2016·Nature Communications·Oscar HernandezValentina Emiliani
Jun 21, 2017·Nature Communications·Orly LibaAdam de la Zerda
Jun 29, 2012·Biomedical Optics Express·Jarett MichaelsonHayden Huang
Jun 29, 2012·Optics Letters·Steven Y Leigh, Jonathan T C Liu
Dec 3, 2010·Optics Letters·Ivan Moreno
Feb 2, 2011·Journal of Biomedical Optics·Daryl LimJerome Mertz
Sep 30, 2015·Biomedical Optics Express·Xiaolin ZhouAndrew Metha
Jul 22, 2017·Science·Carlos Macias-RomeroSylvie Roke
Sep 22, 2011·Optics Express·J MazzaferriS Costantino
Jan 19, 2012·Biomedical Optics Express·Vijay Raj SinghPeter T C So
Oct 10, 2013·Optics Express·Taewoo KimGabriel Popescu
Mar 31, 2018·Journal of Healthcare Engineering·Susana F SilvaAntónio Miguel Morgado
Jan 9, 2019·Frontiers in Cellular Neuroscience·Emiliano RonzittiEirini Papagiakoumou
Aug 20, 2014·Biomedical Optics Express·Xiaolin ZhouAndrew Metha
Jul 15, 2016·Optics Express·Katrin PhilippJürgen W Czarske
May 3, 2014·Optics Letters·Elias KristenssonMarcus Aldén
Feb 9, 2019·Optics Express·Carlos Macias-RomeroSylvie Roke
Mar 21, 2020·Light, Science & Applications·Mo DengGeorge Barbastathis

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