Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain

Nature Biotechnology
Jeffrey N StirmanSpencer L Smith

Abstract

Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to ∼1 mm(2), precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.5 mm(2)) for rapidly reconfigurable simultaneous scanning of widely separated populations of neurons. We custom designed and assembled an optimized scan engine, objective, and two independently positionable, temporally multiplexed excitation pathways. We used this new microscope to measure activity correlations between two cortical visual areas in mice during visual processing.

References

Apr 6, 1990·Science·W DenkW W Webb
Jan 1, 1997·Spatial Vision·D H Brainard
Mar 22, 2001·Biophysical Journal·A Hopt, E Neher
May 27, 2003·Neuron·Valery A Kalatsky, Michael P Stryker
Jun 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christoph StosiekCarsten H Tischbirek
Nov 5, 2003·Nature Biotechnology·Warren R ZipfelWatt W Webb
Apr 22, 2006·International Journal of Psychophysiology : Official Journal of the International Organization of Psychophysiology·Joaquín M Fuster
Nov 16, 2006·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Patrick Theer, Winfried Denk
Dec 5, 2006·Nature Methods·Werner GöbelFritjof Helmchen
Mar 17, 2007·The Journal of Comparative Neurology·Quanxin Wang, Andreas Burkhalter
Sep 6, 2008·Optics Express·Sucbei Moon, Dug Young Kim
May 16, 2009·Nature Protocols·Benjamin JudkewitzMichael Häusser
Jul 22, 2009·Biophysical Journal·Joshua T VogelsteinLiam Paninski
Jun 18, 2010·Journal of Neurophysiology·Joshua T VogelsteinLiam Paninski
Aug 17, 2010·Nature Neuroscience·Spencer L Smith, Michael Häusser
Jan 11, 2011·Nature Methods·Adrian ChengCarlos Portera-Cailliau
Apr 8, 2011·Journal of Neurophysiology·Jonathan D DriscollDavid Kleinfeld
Jul 14, 2011·Biomedical Optics Express·Benjamin F GreweFritjof Helmchen
Sep 22, 2011·Optics Express·Joseph P Zinter, Michael J Levene
Dec 27, 2011·Neuron·James H MarshelEdward M Callaway
Jul 23, 2013·Nature·Tsai-Wen ChenDouglas S Kim
Oct 18, 2013·Frontiers in Neural Circuits·R James CottonAndreas S Tolias
Nov 18, 2014·Nature Neuroscience·Jérôme LecoqMark J Schnitzer
Jun 16, 2015·Optics Express·Philbert S TsaiDavid Kleinfeld

Citations

Sep 24, 2016·ELife·Raghav K Chhetri, Philipp J Keller
Nov 1, 2016·Nature Methods·Robert PrevedelAlipasha Vaziri
Dec 24, 2016·Cell Reports·Tony Hyun KimMark J Schnitzer
Apr 1, 2017·Nature Methods·Weijian Yang, Rafael Yuste
Jun 21, 2017·Science Advances·Jonathan RivnayGeorge G Malliaras
Jan 10, 2018·Frontiers in Neural Circuits·Rune Rasmussen, Keisuke Yonehara
Dec 29, 2017·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Mahesh M Karnani, Jesse Jackson
May 2, 2018·Annual Review of Neuroscience·Siegfried Weisenburger, Alipasha Vaziri
Sep 27, 2018·Journal of Biophotonics·Yao ZhengWei Gong
Jan 24, 2018·Scientific Reports·Yiyi YuSpencer L Smith
Aug 30, 2016·Nature Neuroscience·Michael Z Lin, Mark J Schnitzer
Aug 30, 2016·Nature Neuroscience·Na JiSpencer L Smith
Aug 30, 2016·Nature Neuroscience·Kenneth D HarrisSpencer L Smith
Jul 25, 2019·Lasers in Surgery and Medicine·Hoonchul ChangKi Hean Kim
Aug 5, 2017·Annual Review of Neuroscience·Tania A SeabrookAndrew D Huberman
Jan 4, 2018·Biomedical Optics Express·Christopher A WerleyAdam E Cohen
Jan 15, 2020·ELife·Andres de GrootTycho M Hoogland
Feb 2, 2020·Brain Structure & Function·Christian R LeeDavid J Margolis
Dec 21, 2016·The Journal of Physiology·Carsten H TischbirekArthur Konnerth
Sep 16, 2017·BMC Biology·Nicholas James Sofroniew
Oct 2, 2018·Optics Letters·Qinglei HuShaoqun Zeng
Feb 2, 2019·Scientific Reports·Leila GhanbariSuhasa B Kodandaramaiah
Dec 5, 2019·Biomedical Optics Express·Kevin T TakasakiJack Waters
May 15, 2020·Optics Express·Jun JiangMartin C Fischer
Sep 5, 2018·Nature Communications·Shin-Ichiro TeradaMasanori Matsuzaki
Jan 9, 2019·Frontiers in Cellular Neuroscience·Emiliano RonzittiEirini Papagiakoumou
Apr 4, 2019·Nature Communications·Leila GhanbariSuhasa B Kodandaramaiah
Feb 12, 2020·Nature Methods·Devin R BeaulieuJerome Mertz
Jun 4, 2019·Frontiers in Synaptic Neuroscience·Jean-Sébastien Jouhanneau, James F A Poulet
Dec 5, 2019·Light, Science & Applications·Mengke YangHongbo Jia

Methods Mentioned

BETA
transgenic

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Related Papers

IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society
A DiasproM Robello
© 2021 Meta ULC. All rights reserved