Wide field of view multifocal scanning microscopy with sparse sampling

Journal of Biomedical Optics
Jie Wang, Jigang Wu

Abstract

We propose to use sparsely sampled line scans with a sparsity-based reconstruction method to obtain images in a wide field of view (WFOV) multifocal scanning microscope. In the WFOV microscope, we used a holographically generated irregular focus grid to scan the sample in one dimension and then reconstructed the sample image from line scans by measuring the transmission of the foci through the sample during scanning. The line scans were randomly spaced with average spacing larger than the Nyquist sampling requirement, and the image was recovered with sparsity-based reconstruction techniques. With this scheme, the acquisition data can be significantly reduced and the restriction for equally spaced foci positions can be removed, indicating simpler experimental requirement. We built a prototype system and demonstrated the effectiveness of the reconstruction by recovering microscopic images of a U.S. Air Force target and an onion skin cell microscope slide with 40, 60, and 80% missing data with respect to the Nyquist sampling requirement.

References

Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·W XuH J Kreuzer
Oct 17, 2006·International Journal of Surgical Pathology·Marcial García RojoManuel Carbajo Vicente
Dec 8, 2009·Archives of Pathology & Laboratory Medicine·David C WilburWolfgang Klietmann
Jul 3, 2010·Optics Letters·Jigang WuChanghuei Yang
Jul 20, 2010·Optics Express·Jigang WuChanghuei Yang
Oct 14, 2010·Optics Express·Evgeniy LebedMirza Faisal Beg
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Guoan ZhengChanghuei Yang
Oct 13, 2011·Biomedical Optics Express·Mei YoungMarinko V Sarunic
Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Vincent StuderMaxime Dahan
Sep 23, 2014·Nature Photonics·Guoan ZhengChanghuei Yang
May 20, 2015·Virchows Archiv : an International Journal of Pathology·Yibo ZhangAydogan Ozcan

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